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Assessing the immune status of critically ill trauma patients by flow cytometry.

Publication ,  Journal Article
Kuethe, JW; Mintz-Cole, R; Johnson, BL; Midura, EF; Caldwell, CC; Schneider, BSP
Published in: Nurs Res
2014

BACKGROUND: Unintentional injury or trauma remains the leading cause of death among young adults. About one fifth of these trauma patients require care in an intensive care unit (ICU) because of severity of injuries and comorbidities. Patients hospitalized in an ICU are at increased risk for nosocomial infections, such as urinary tract infections, pneumonia, bacteremia, and wound infections. Many of these patients will develop sepsis or septic shock, and some will progress to multiple organ failure and death. The balance between the proinflammatory and counterinflammatory immune response appears to be a driving factor in this progression. At present, there is no proposed method for the timely detection of the immune status in trauma patients, making rational decisions to use immune-altering therapies difficult. OBJECTIVE: We demonstrate that flow cytometry, with its capabilities to characterize and/or enumerate (a) leukocyte subtypes, (b) leukocyte activation markers, (c) leukocyte-derived cytokines and microvesicles, and (d) leukocyte function is well suited to monitor the immune status of critically ill trauma patients. METHODS: Information for the review was obtained from the extant literature. DISCUSSION: We suggest that flow cytometry is a research method that might aid nurse scientists in investigating the immune status of critically ill patients, the recovery status of conditions such as hemorrhagic shock and tissue injury and the relationship between cancer disease progression and symptoms. Therefore, flow cytometry has the potential to broaden nursing research priority areas so that a comprehensive approach to understanding the cellular response is attained.

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Published In

Nurs Res

DOI

EISSN

1538-9847

Publication Date

2014

Volume

63

Issue

6

Start / End Page

426 / 434

Location

United States

Related Subject Headings

  • Sepsis
  • Reactive Oxygen Species
  • Nursing
  • Lymphocytes
  • Killer Cells, Natural
  • Immunity, Innate
  • Humans
  • Flow Cytometry
  • Cytokines
  • Critical Illness
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kuethe, J. W., Mintz-Cole, R., Johnson, B. L., Midura, E. F., Caldwell, C. C., & Schneider, B. S. P. (2014). Assessing the immune status of critically ill trauma patients by flow cytometry. Nurs Res, 63(6), 426–434. https://doi.org/10.1097/NNR.0000000000000061
Kuethe, Joshua W., Rachael Mintz-Cole, Bobby L. Johnson, Emily F. Midura, Charles C. Caldwell, and Barbara St Pierre Schneider. “Assessing the immune status of critically ill trauma patients by flow cytometry.Nurs Res 63, no. 6 (2014): 426–34. https://doi.org/10.1097/NNR.0000000000000061.
Kuethe JW, Mintz-Cole R, Johnson BL, Midura EF, Caldwell CC, Schneider BSP. Assessing the immune status of critically ill trauma patients by flow cytometry. Nurs Res. 2014;63(6):426–34.
Kuethe, Joshua W., et al. “Assessing the immune status of critically ill trauma patients by flow cytometry.Nurs Res, vol. 63, no. 6, 2014, pp. 426–34. Pubmed, doi:10.1097/NNR.0000000000000061.
Kuethe JW, Mintz-Cole R, Johnson BL, Midura EF, Caldwell CC, Schneider BSP. Assessing the immune status of critically ill trauma patients by flow cytometry. Nurs Res. 2014;63(6):426–434.

Published In

Nurs Res

DOI

EISSN

1538-9847

Publication Date

2014

Volume

63

Issue

6

Start / End Page

426 / 434

Location

United States

Related Subject Headings

  • Sepsis
  • Reactive Oxygen Species
  • Nursing
  • Lymphocytes
  • Killer Cells, Natural
  • Immunity, Innate
  • Humans
  • Flow Cytometry
  • Cytokines
  • Critical Illness