Lactate Is a Natural Suppressor of RLR Signaling by Targeting MAVS.
RLR-mediated type I IFN production plays a pivotal role in elevating host immunity for viral clearance and cancer immune surveillance. Here, we report that glycolysis, which is inactivated during RLR activation, serves as a barrier to impede type I IFN production upon RLR activation. RLR-triggered MAVS-RIG-I recognition hijacks hexokinase binding to MAVS, leading to the impairment of hexokinase mitochondria localization and activation. Lactate serves as a key metabolite responsible for glycolysis-mediated RLR signaling inhibition by directly binding to MAVS transmembrane (TM) domain and preventing MAVS aggregation. Notably, lactate restoration reverses increased IFN production caused by lactate deficiency. Using pharmacological and genetic approaches, we show that lactate reduction by lactate dehydrogenase A (LDHA) inactivation heightens type I IFN production to protect mice from viral infection. Our study establishes a critical role of glycolysis-derived lactate in limiting RLR signaling and identifies MAVS as a direct sensor of lactate, which functions to connect energy metabolism and innate immunity.
Duke Scholars
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Related Subject Headings
- Transfection
- Signal Transduction
- Receptors, Immunologic
- Receptors, Cell Surface
- RAW 264.7 Cells
- Mice, Transgenic
- Mice, Inbred C57BL
- Mice
- Male
- Lactic Acid
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transfection
- Signal Transduction
- Receptors, Immunologic
- Receptors, Cell Surface
- RAW 264.7 Cells
- Mice, Transgenic
- Mice, Inbred C57BL
- Mice
- Male
- Lactic Acid