Selection and Screening of Antibody Phage Display Libraries
The following sections are included: Introduction Antibody Gene Rearrangement and Variable Region Sequence Diversity Antibody architecture and germline gene segment recombination Variable region sequence diversity and structure guiding library design Amplification of variable region sequence repertoires Antibody sequence databases Antibody Display Libraries in Ff Bacteriophage Phage biology and early use as a display system Variation of display format and control of display level Coat protein display position Display format, scFv vs. Fab Signal peptide and vector design Control of functional display level and valency Maximizing library size Assessment of diversity Representative large non-immune libraries Library Selection Antigen immobilization Overview of phage display selection Advantages and successes of phage display Species cross-reactivity Homolog binders Rare epitopes Exquisite specificity Cell surface antigens Toxins High-throughput generation of reagents for proteomics Advances in selection technology Library Screening Primary screening methods Secondary screening methods High-throughput antibody purification Future Applications of Antibody Phage Display Antibody phage display in the proteomics arena Quality protein reagents Affinity maturation of antibody outputs Antibody format and production Conclusions References.
