A homogeneous fluorescence anisotropy assay for measuring transglutaminase 2 activity.
Publication
, Journal Article
Kenniston, JA; Conley, GP; Sexton, DJ; Nixon, AE
Published in: Anal Biochem
May 1, 2013
Transglutaminases catalyze the covalent linkage of protein polypeptides through their glutamine and lysine side chains. Tissue transglutaminase 2 (TG2) has been of particular interest given its potential role in several disorders, including a variety of cancers and neurodegenerative diseases. Here we report a biochemical assay that monitors TG2 activity by following an increase in the fluorescence anisotropy of a fluorescein-labeled substrate peptide as it conjugates to a bovine serum albumin (BSA) cosubstrate of larger hydrodynamic mass. The resulting homogeneous assay is sensitive to low TG2 concentrations (pM range) and is readily adapted to higher throughput formats.
Duke Scholars
Published In
Anal Biochem
DOI
EISSN
1096-0309
Publication Date
May 1, 2013
Volume
436
Issue
1
Start / End Page
13 / 15
Location
United States
Related Subject Headings
- Transglutaminases
- Serum Albumin, Bovine
- Protein Glutamine gamma Glutamyltransferase 2
- Peptides
- Humans
- GTP-Binding Proteins
- Fluorescence
- Fluorescein
- Enzyme Activation
- Cattle
Citation
APA
Chicago
ICMJE
MLA
NLM
Kenniston, J. A., Conley, G. P., Sexton, D. J., & Nixon, A. E. (2013). A homogeneous fluorescence anisotropy assay for measuring transglutaminase 2 activity. Anal Biochem, 436(1), 13–15. https://doi.org/10.1016/j.ab.2013.01.016
Kenniston, Jon A., Gregory P. Conley, Daniel J. Sexton, and Andrew E. Nixon. “A homogeneous fluorescence anisotropy assay for measuring transglutaminase 2 activity.” Anal Biochem 436, no. 1 (May 1, 2013): 13–15. https://doi.org/10.1016/j.ab.2013.01.016.
Kenniston JA, Conley GP, Sexton DJ, Nixon AE. A homogeneous fluorescence anisotropy assay for measuring transglutaminase 2 activity. Anal Biochem. 2013 May 1;436(1):13–5.
Kenniston, Jon A., et al. “A homogeneous fluorescence anisotropy assay for measuring transglutaminase 2 activity.” Anal Biochem, vol. 436, no. 1, May 2013, pp. 13–15. Pubmed, doi:10.1016/j.ab.2013.01.016.
Kenniston JA, Conley GP, Sexton DJ, Nixon AE. A homogeneous fluorescence anisotropy assay for measuring transglutaminase 2 activity. Anal Biochem. 2013 May 1;436(1):13–15.
Published In
Anal Biochem
DOI
EISSN
1096-0309
Publication Date
May 1, 2013
Volume
436
Issue
1
Start / End Page
13 / 15
Location
United States
Related Subject Headings
- Transglutaminases
- Serum Albumin, Bovine
- Protein Glutamine gamma Glutamyltransferase 2
- Peptides
- Humans
- GTP-Binding Proteins
- Fluorescence
- Fluorescein
- Enzyme Activation
- Cattle