Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids.
Publication
, Chapter
Yang, H; Lan, L
2022
RNA:DNA hybrids not only function in various physiological cellular processes but also represent a threat to genome integrity. The methyl-5-cytosine (m5C) marked RNA:DNA hybrids containing the m5C modified RNA strand add another layer of regulation to the R-loop structure. Here we describe the detection of m5C modifications in the context of RNA:DNA hybrids using immunostaining, and identification of specific binding partners of m5C marked RNA:DNA hybrids using pull-down method.
Duke Scholars
DOI
Publication Date
2022
Volume
2528
Start / End Page
271 / 276
Related Subject Headings
- RNA
- Developmental Biology
- DNA
- Cytosine
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Yang, H., & Lan, L. (2022). Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. (Vol. 2528, pp. 271–276). https://doi.org/10.1007/978-1-0716-2477-7_17
Yang, Haibo, and Li Lan. “Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids.,” 2528:271–76, 2022. https://doi.org/10.1007/978-1-0716-2477-7_17.
Yang H, Lan L. Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. In 2022. p. 271–6.
Yang, Haibo, and Li Lan. Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. Vol. 2528, 2022, pp. 271–76. Pubmed, doi:10.1007/978-1-0716-2477-7_17.
Yang H, Lan L. Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. 2022. p. 271–276.
DOI
Publication Date
2022
Volume
2528
Start / End Page
271 / 276
Related Subject Headings
- RNA
- Developmental Biology
- DNA
- Cytosine
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences