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SIRT6 facilitates directional telomere movement upon oxidative damage.

Publication ,  Journal Article
Gao, Y; Tan, J; Jin, J; Ma, H; Chen, X; Leger, B; Xu, J; Spagnol, ST; Dahl, KN; Levine, AS; Liu, Y; Lan, L
Published in: Sci Rep
March 29, 2018

Oxidative damage to telomeres leads to telomere attrition and genomic instability, resulting in poor cell viability. Telomere dynamics contribute to the maintenance of telomere integrity; however, whether oxidative damage induces telomere movement and how telomere mobility is regulated remain poorly understood. Here, we show that oxidative damage at telomeres triggers directional telomere movement. The presence of the human Sir2 homolog, Sirtuin 6 (SIRT6) is required for oxidative damage-induced telomeric movement. SIRT6 knock out (KO) cells show neither damage-induced telomere movement nor chromatin decondensation at damaged telomeres; both are observed in wild type (WT) cells. A deacetylation mutant of SIRT6 increases damage-induced telomeric movement in SIRT6 KO cells as well as WT SIRT6. SIRT6 recruits the chromatin-remodeling protein SNF2H to damaged telomeres, which appears to promote chromatin decondensation independent of its deacetylase activity. Together, our results suggest that SIRT6 plays a role in the regulation of telomere movement upon oxidative damage, shedding new light onto the function of SIRT6 in telomere maintenance.

Duke Scholars

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 29, 2018

Volume

8

Issue

1

Start / End Page

5407

Location

England

Related Subject Headings

  • Telomere
  • Sirtuins
  • RNA, Guide, Kinetoplastida
  • Oxidative Stress
  • Microscopy, Fluorescence
  • Humans
  • Gene Editing
  • DNA Repair
  • DNA Damage
  • Chromosomal Proteins, Non-Histone
 

Citation

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Gao, Y., Tan, J., Jin, J., Ma, H., Chen, X., Leger, B., … Lan, L. (2018). SIRT6 facilitates directional telomere movement upon oxidative damage. Sci Rep, 8(1), 5407. https://doi.org/10.1038/s41598-018-23602-0
Gao, Ying, Jun Tan, Jingyi Jin, Hongqiang Ma, Xiukai Chen, Brittany Leger, Jianquan Xu, et al. “SIRT6 facilitates directional telomere movement upon oxidative damage.Sci Rep 8, no. 1 (March 29, 2018): 5407. https://doi.org/10.1038/s41598-018-23602-0.
Gao Y, Tan J, Jin J, Ma H, Chen X, Leger B, et al. SIRT6 facilitates directional telomere movement upon oxidative damage. Sci Rep. 2018 Mar 29;8(1):5407.
Gao, Ying, et al. “SIRT6 facilitates directional telomere movement upon oxidative damage.Sci Rep, vol. 8, no. 1, Mar. 2018, p. 5407. Pubmed, doi:10.1038/s41598-018-23602-0.
Gao Y, Tan J, Jin J, Ma H, Chen X, Leger B, Xu J, Spagnol ST, Dahl KN, Levine AS, Liu Y, Lan L. SIRT6 facilitates directional telomere movement upon oxidative damage. Sci Rep. 2018 Mar 29;8(1):5407.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 29, 2018

Volume

8

Issue

1

Start / End Page

5407

Location

England

Related Subject Headings

  • Telomere
  • Sirtuins
  • RNA, Guide, Kinetoplastida
  • Oxidative Stress
  • Microscopy, Fluorescence
  • Humans
  • Gene Editing
  • DNA Repair
  • DNA Damage
  • Chromosomal Proteins, Non-Histone