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Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein.

Publication ,  Journal Article
Benitez, A; Yuan, F; Nakajima, S; Wei, L; Qian, L; Myers, R; Hu, JJ; Lan, L; Zhang, Y
Published in: Nucleic Acids Res
February 2014

MUS81-EME1 is a DNA endonuclease involved in replication-coupled repair of DNA interstrand cross-links (ICLs). A prevalent hypothetical role of MUS81-EME1 in ICL repair is to unhook the damage by incising the leading strand at the 3' side of an ICL lesion. In this study, we report that purified MUS81-EME1 incises DNA at the 5' side of a psoralen ICL residing in fork structures. Intriguingly, ICL repair protein, Fanconi anemia complementation group A protein (FANCA), greatly enhances MUS81-EME1-mediated ICL incision. On the contrary, FANCA exhibits a two-phase incision regulation when DNA is undamaged or the damage affects only one DNA strand. Studies using truncated FANCA proteins indicate that both the N- and C-moieties of the protein are required for the incision regulation. Using laser-induced psoralen ICL formation in cells, we find that FANCA interacts with and recruits MUS81 to ICL lesions. This report clarifies the incision specificity of MUS81-EME1 on ICL damage and establishes that FANCA regulates the incision activity of MUS81-EME1 in a damage-dependent manner.

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Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

February 2014

Volume

42

Issue

3

Start / End Page

1671 / 1683

Location

England

Related Subject Headings

  • Methoxsalen
  • Humans
  • Fanconi Anemia Complementation Group A Protein
  • Endonucleases
  • Endodeoxyribonucleases
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA Damage
  • Cross-Linking Reagents
  • 41 Environmental sciences
 

Citation

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Benitez, A., Yuan, F., Nakajima, S., Wei, L., Qian, L., Myers, R., … Zhang, Y. (2014). Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein. Nucleic Acids Res, 42(3), 1671–1683. https://doi.org/10.1093/nar/gkt975
Benitez, Anaid, Fenghua Yuan, Satoshi Nakajima, Leizhen Wei, Liangyue Qian, Richard Myers, Jennifer J. Hu, Li Lan, and Yanbin Zhang. “Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein.Nucleic Acids Res 42, no. 3 (February 2014): 1671–83. https://doi.org/10.1093/nar/gkt975.
Benitez A, Yuan F, Nakajima S, Wei L, Qian L, Myers R, et al. Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein. Nucleic Acids Res. 2014 Feb;42(3):1671–83.
Benitez, Anaid, et al. “Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein.Nucleic Acids Res, vol. 42, no. 3, Feb. 2014, pp. 1671–83. Pubmed, doi:10.1093/nar/gkt975.
Benitez A, Yuan F, Nakajima S, Wei L, Qian L, Myers R, Hu JJ, Lan L, Zhang Y. Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein. Nucleic Acids Res. 2014 Feb;42(3):1671–1683.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

February 2014

Volume

42

Issue

3

Start / End Page

1671 / 1683

Location

England

Related Subject Headings

  • Methoxsalen
  • Humans
  • Fanconi Anemia Complementation Group A Protein
  • Endonucleases
  • Endodeoxyribonucleases
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA Damage
  • Cross-Linking Reagents
  • 41 Environmental sciences