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Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage.

Publication ,  Journal Article
Yoshimura, M; Kohzaki, M; Nakamura, J; Asagoshi, K; Sonoda, E; Hou, E; Prasad, R; Wilson, SH; Tano, K; Yasui, A; Lan, L; Seki, M; Wood, RD ...
Published in: Mol Cell
October 6, 2006

Base excision repair (BER) plays an essential role in protecting cells from mutagenic base damage caused by oxidative stress, hydrolysis, and environmental factors. POLQ is a DNA polymerase, which appears to be involved in translesion DNA synthesis (TLS) past base damage. We disrupted POLQ, and its homologs HEL308 and POLN in chicken DT40 cells, and also created polq/hel308 and polq/poln double mutants. We found that POLQ-deficient mutants exhibit hypersensitivity to oxidative base damage induced by H(2)O(2), but not to UV or cisplatin. Surprisingly, this phenotype was synergistically increased by concomitant deletion of the major BER polymerase, POLbeta. Moreover, extracts from a polq null mutant cell line show reduced BER activity, and POLQ, like POLbeta, accumulated rapidly at sites of base damage. Accordingly, POLQ and POLbeta share an overlapping function in the repair of oxidative base damage. Taken together, these results suggest a role for vertebrate POLQ in BER.

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Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

October 6, 2006

Volume

24

Issue

1

Start / End Page

115 / 125

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Protein Structure, Tertiary
  • Oxidative Stress
  • Mutation
  • Hydrogen Peroxide
  • Gene Deletion
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Repair
  • DNA Polymerase beta
 

Citation

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Yoshimura, M., Kohzaki, M., Nakamura, J., Asagoshi, K., Sonoda, E., Hou, E., … Takeda, S. (2006). Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell, 24(1), 115–125. https://doi.org/10.1016/j.molcel.2006.07.032
Yoshimura, Michio, Masaoki Kohzaki, Jun Nakamura, Kenjiro Asagoshi, Eiichiro Sonoda, Esther Hou, Rajendra Prasad, et al. “Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage.Mol Cell 24, no. 1 (October 6, 2006): 115–25. https://doi.org/10.1016/j.molcel.2006.07.032.
Yoshimura M, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, et al. Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell. 2006 Oct 6;24(1):115–25.
Yoshimura, Michio, et al. “Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage.Mol Cell, vol. 24, no. 1, Oct. 2006, pp. 115–25. Pubmed, doi:10.1016/j.molcel.2006.07.032.
Yoshimura M, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde J-M, Hochegger H, Okada T, Hiraoka M, Takeda S. Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell. 2006 Oct 6;24(1):115–125.
Journal cover image

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

October 6, 2006

Volume

24

Issue

1

Start / End Page

115 / 125

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Protein Structure, Tertiary
  • Oxidative Stress
  • Mutation
  • Hydrogen Peroxide
  • Gene Deletion
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Repair
  • DNA Polymerase beta