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The ACF1 complex is required for DNA double-strand break repair in human cells.

Publication ,  Journal Article
Lan, L; Ui, A; Nakajima, S; Hatakeyama, K; Hoshi, M; Watanabe, R; Janicki, SM; Ogiwara, H; Kohno, T; Kanno, S-I; Yasui, A
Published in: Mol Cell
December 22, 2010

DNA double-strand breaks (DSBs) are repaired via nonhomologous end-joining (NHEJ) or homologous recombination (HR), but cellular repair processes remain elusive. We show here that the ATP-dependent chromatin-remodeling factors, ACF1 and SNF2H, accumulate rapidly at DSBs and are required for DSB repair in human cells. If the expression of ACF1 or SNF2H is suppressed, cells become extremely sensitive to X-rays and chemical treatments producing DSBs, and DSBs remain unrepaired. ACF1 interacts directly with KU70 and is required for the accumulation of KU proteins at DSBs. The KU70/80 complex becomes physically more associated with the chromatin-remodeling factors of the CHRAC complex, which includes ACF1, SNF2H, CHRAC15, and CHRAC17, after treatments producing DSBs. Furthermore, the frequency of NHEJ as well as HR induced by DSBs in chromosomal DNA is significantly decreased in cells depleted of either of these factors. Thus, ACF1 and its complexes play important roles in DSBs repair.

Duke Scholars

Published In

Mol Cell

DOI

EISSN

1097-4164

Publication Date

December 22, 2010

Volume

40

Issue

6

Start / End Page

976 / 987

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Transcription Factors
  • Nucleoproteins
  • Ku Autoantigen
  • Kinetics
  • Humans
  • Green Fluorescent Proteins
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA Repair
 

Citation

APA
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ICMJE
MLA
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Lan, L., Ui, A., Nakajima, S., Hatakeyama, K., Hoshi, M., Watanabe, R., … Yasui, A. (2010). The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell, 40(6), 976–987. https://doi.org/10.1016/j.molcel.2010.12.003
Lan, Li, Ayako Ui, Satoshi Nakajima, Kotomi Hatakeyama, Mikiko Hoshi, Reiko Watanabe, Susan M. Janicki, et al. “The ACF1 complex is required for DNA double-strand break repair in human cells.Mol Cell 40, no. 6 (December 22, 2010): 976–87. https://doi.org/10.1016/j.molcel.2010.12.003.
Lan L, Ui A, Nakajima S, Hatakeyama K, Hoshi M, Watanabe R, et al. The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell. 2010 Dec 22;40(6):976–87.
Lan, Li, et al. “The ACF1 complex is required for DNA double-strand break repair in human cells.Mol Cell, vol. 40, no. 6, Dec. 2010, pp. 976–87. Pubmed, doi:10.1016/j.molcel.2010.12.003.
Lan L, Ui A, Nakajima S, Hatakeyama K, Hoshi M, Watanabe R, Janicki SM, Ogiwara H, Kohno T, Kanno S-I, Yasui A. The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell. 2010 Dec 22;40(6):976–987.
Journal cover image

Published In

Mol Cell

DOI

EISSN

1097-4164

Publication Date

December 22, 2010

Volume

40

Issue

6

Start / End Page

976 / 987

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Transcription Factors
  • Nucleoproteins
  • Ku Autoantigen
  • Kinetics
  • Humans
  • Green Fluorescent Proteins
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA Repair