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[Motilin receptor expression in the interstitial cells of Cajal].

Publication ,  Journal Article
Xu, W-D; Jiang, X; Lan, L; Tong, H-X; Wang, C-H; Wang, B-X
Published in: Zhonghua Er Ke Za Zhi
April 2011

OBJECTIVE: To look for the evidences of motilin receptor expression on interstitial cells of Cajal (ICC) of the rabbit. METHOD: Smooth muscle segments with ICC were isolated from the small intestine of 10-day old rabbits. The tissue segments equilibrated in Ca(2+)-free Hanks' solution were dispersed with an enzyme solution containing collagenase type II and then Ficoll density centrifugation was used to dissociate ICC. The cells were suspended and cultured in the M199 medium. The c-kit antibody was applied to distinguish the cultured ICC. The motilin receptor was identified by immunocytochemical assay with GPR38 antibody, c-kit antibody and hoechst 33342 combined to label ICC. Cells cultured for a few days were sorted for ICC with c-kit stained green fluorescent through flow cytometry. The total RNA and proteins extracted from the sorted ICC were respectively used to verify motilin receptor on the ICC by reverse-transcriptase polymerase chain reaction (RT-PCR) and Western blotting. RESULT: We had successfully dissociated and cultured ICC of rabbit small intestine in vitro. Fluorescent staining with c-kit antibody confirmed that the culture ICC was successful. Triple-labeled immunofluorescent staining had detected the motilin receptor on membrane of ICC. Flow cytometry analysis showed that the ratio of c-kit positive cell in the cultured cells was 64.3%. The number of sorted ICC was 6.7 × 10(5) and 5.6 × 10(6). The results of RT-PCR and Western blot confirmed that the ICC had motilin receptor expression. CONCLUSION: Our study demonstrated presence of motilin receptor on ICC of the rabbit. The present results may suggest that ICC play an important role in gastrointestinal movement induced by motilin.

Duke Scholars

Published In

Zhonghua Er Ke Za Zhi

ISSN

0578-1310

Publication Date

April 2011

Volume

49

Issue

4

Start / End Page

255 / 260

Location

China

Related Subject Headings

  • Receptors, Neuropeptide
  • Receptors, Gastrointestinal Hormone
  • Rabbits
  • Intestine, Small
  • Interstitial Cells of Cajal
  • Cells, Cultured
  • Animals
 

Citation

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ICMJE
MLA
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Xu, W.-D., Jiang, X., Lan, L., Tong, H.-X., Wang, C.-H., & Wang, B.-X. (2011). [Motilin receptor expression in the interstitial cells of Cajal]. Zhonghua Er Ke Za Zhi, 49(4), 255–260.
Xu, Wen-da, Xun Jiang, Li Lan, Hai-Xia Tong, Chun-Hui Wang, and Bao-Xi Wang. “[Motilin receptor expression in the interstitial cells of Cajal].Zhonghua Er Ke Za Zhi 49, no. 4 (April 2011): 255–60.
Xu W-D, Jiang X, Lan L, Tong H-X, Wang C-H, Wang B-X. [Motilin receptor expression in the interstitial cells of Cajal]. Zhonghua Er Ke Za Zhi. 2011 Apr;49(4):255–60.
Xu, Wen-da, et al. “[Motilin receptor expression in the interstitial cells of Cajal].Zhonghua Er Ke Za Zhi, vol. 49, no. 4, Apr. 2011, pp. 255–60.
Xu W-D, Jiang X, Lan L, Tong H-X, Wang C-H, Wang B-X. [Motilin receptor expression in the interstitial cells of Cajal]. Zhonghua Er Ke Za Zhi. 2011 Apr;49(4):255–260.

Published In

Zhonghua Er Ke Za Zhi

ISSN

0578-1310

Publication Date

April 2011

Volume

49

Issue

4

Start / End Page

255 / 260

Location

China

Related Subject Headings

  • Receptors, Neuropeptide
  • Receptors, Gastrointestinal Hormone
  • Rabbits
  • Intestine, Small
  • Interstitial Cells of Cajal
  • Cells, Cultured
  • Animals