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The effects of osmotic pressure changing on connexins expression in cultured rat hypothalamic astrocytes and neurons

Publication ,  Journal Article
Cao, R; Lan, L; Duan, L; Zhang, P; Xiong, YF; Gao, B; Rao, ZR
Published in: Acta Anatomica Sinica
August 1, 2006

Objective: To investigate the effects of osmotic pressure changing on connexin43 (Cx43) and Cx32 expression in cultured rat hypothalamic astrocytes and neurons. Methods: By culturing astrocytes and neurons fr om newborn rats, these cells were divided to two groups. The cells in first group were moved from isotonic culture medium to hypertonic culture medium and incubated for 1, 3, 5, 10 and 15 min respectively, then, fixed by 4% paraformaldehyde. The cells in second group were moved from hypertonic culture medium to isotonic culture medium and incubated for 1, 3, 5 and 10 min respectively, they were fixed too. These cells were stained by anti-Cx43 or anti-Cx32 immunofluorescent method and observed under confocal microscope. Results: In first group, the expression of Cx43 in creased then incubation for 1 min, at 3 min peaked, then decreased, at 15 min backed to normal level. The expression of Cx32 peaked at 5 min, later decreased, at 15 min backed to normal level. In second group, the expression of Cx43 also increased after incubation for 1 min, at 3 min peaked, then decreased, at 10 min backed to normal level. Cx32 expression peaked at 5 min, then decreased, at 10 min backed to normal level. The peak of Cx43 expression was early than that of Cx32. Conclusion: Cx43 and Cx32 might be involved in osmoregulation.

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Published In

Acta Anatomica Sinica

ISSN

0529-1356

Publication Date

August 1, 2006

Volume

37

Issue

4

Start / End Page

371 / 375

Related Subject Headings

  • Anatomy & Morphology
  • 1103 Clinical Sciences
 

Citation

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MLA
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Cao, R., Lan, L., Duan, L., Zhang, P., Xiong, Y. F., Gao, B., & Rao, Z. R. (2006). The effects of osmotic pressure changing on connexins expression in cultured rat hypothalamic astrocytes and neurons. Acta Anatomica Sinica, 37(4), 371–375.
Cao, R., L. Lan, L. Duan, P. Zhang, Y. F. Xiong, B. Gao, and Z. R. Rao. “The effects of osmotic pressure changing on connexins expression in cultured rat hypothalamic astrocytes and neurons.” Acta Anatomica Sinica 37, no. 4 (August 1, 2006): 371–75.
Cao R, Lan L, Duan L, Zhang P, Xiong YF, Gao B, et al. The effects of osmotic pressure changing on connexins expression in cultured rat hypothalamic astrocytes and neurons. Acta Anatomica Sinica. 2006 Aug 1;37(4):371–5.
Cao, R., et al. “The effects of osmotic pressure changing on connexins expression in cultured rat hypothalamic astrocytes and neurons.” Acta Anatomica Sinica, vol. 37, no. 4, Aug. 2006, pp. 371–75.
Cao R, Lan L, Duan L, Zhang P, Xiong YF, Gao B, Rao ZR. The effects of osmotic pressure changing on connexins expression in cultured rat hypothalamic astrocytes and neurons. Acta Anatomica Sinica. 2006 Aug 1;37(4):371–375.

Published In

Acta Anatomica Sinica

ISSN

0529-1356

Publication Date

August 1, 2006

Volume

37

Issue

4

Start / End Page

371 / 375

Related Subject Headings

  • Anatomy & Morphology
  • 1103 Clinical Sciences