Effect of carbenoxolone on behavior and electroencephalogram of PTZ-kindled epileptic rats

Objective: To explore the effect of carbenoxolone, a gap junction blocker, on seizure and electroencephalogram (EEG) of PTZ-kindled epileptic rats. Methods: Circled digit one Of the 15 adult male Sprague-Dawley rats used in the study, 10 were intraperitoneally injected with PTZ [35 mg/(kg·d)] to established epilepsy model of kindled rats, and then were intraperitoneally injected with carbenoxolone (CBX, 10 mg/kg) or normal saline (NS). The other 5 rats were in control group. The changes of seizures were analyzed within K group, KCBX group and NS group. Circled digit two After putting electrode in the hippocampi and amydaloid nucleus, epilepsy model of kindled rats was established in 7 rats. The changes of brain wave induced by injection of CBX in the PTZ-kindled epileptic rats were analyzed. Results: Circled digit one Spontaneous seizures appeared in PTZ-kindled epileptic rats. The number of accumulative times of seizures in KCBX group rats was fewer than that in K group rats. Circled digit two EEG recordings of the PTZ-kindled epileptic rats displayed various seizure waveforms. The rhythmic spikes or the spike and wave complexes were obvious properties of the PTZ-kindled epileptic rats. After treated with CBX, the rhythmic spike and wave complexes decreased; meanwhile, the amplitude of background rhythm enlarged and slow waves increased. Conclusion: Gap junction and hemichannels are closely involved in epileptogenesis. Gap junction blocker may have an anti-epileptic potential.

Duke Scholars

Author Li Lan Molecular Genetics and Microbiology