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The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast.

Publication ,  Journal Article
Zhu, J; Jia, Z-W; Xia, C-Y; Gao, X-D
Published in: Fungal genetics and biology : FG & B
November 2020

Tos7 (Yol019w) is a Sur7/PalI family transmembrane protein in the budding yeast Saccharomyces cerevisiae. Since the deletion of TOS7 did not affect growth or cell morphology, the cellular roles of Tos7 have not been established previously. Here, we show that high-copy TOS7 expression suppressed the growth defect of the secretion-defective RGA1-C term-overexpressing mutant and sec15-1 mutant. Moreover, Tos7 physically interacted with Boi2 and the Rho GTPase Rho3, two key regulators of exocyst assembly, suggesting that Tos7 plays a role in secretion. We also show that the deletion of TOS7 rendered the cells more sensitive to the cell wall-disrupting agents Congo red and calcofluor white while high-copy TOS7 expression had an opposite effect, suggesting that Tos7 affects cell wall organization. Finally, we show that Tos7 localized to punctate patches on the plasma membrane that were largely co-localized with the plasma membrane microdomains named MCC (membrane compartment of Can1). Together, these results suggest that Tos7 contributes to cell surface-related functions. Tos7 is likely an auxiliary component of MCC/eisosome that specifically interacts with the secretory pathway.

Duke Scholars

Published In

Fungal genetics and biology : FG & B

DOI

EISSN

1096-0937

ISSN

1087-1845

Publication Date

November 2020

Volume

144

Start / End Page

103467

Related Subject Headings

  • rho GTP-Binding Proteins
  • Vesicular Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Microbiology
  • Membrane Proteins
  • Gene Expression Regulation, Fungal
  • Exocytosis
  • Cell Wall
  • Amino Acid Transport Systems, Basic
 

Citation

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MLA
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Zhu, J., Jia, Z.-W., Xia, C.-Y., & Gao, X.-D. (2020). The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast. Fungal Genetics and Biology : FG & B, 144, 103467. https://doi.org/10.1016/j.fgb.2020.103467
Zhu, Jing, Zhi-Wen Jia, Chen-Yang Xia, and Xiang-Dong Gao. “The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast.Fungal Genetics and Biology : FG & B 144 (November 2020): 103467. https://doi.org/10.1016/j.fgb.2020.103467.
Zhu J, Jia Z-W, Xia C-Y, Gao X-D. The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast. Fungal genetics and biology : FG & B. 2020 Nov;144:103467.
Zhu, Jing, et al. “The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast.Fungal Genetics and Biology : FG & B, vol. 144, Nov. 2020, p. 103467. Epmc, doi:10.1016/j.fgb.2020.103467.
Zhu J, Jia Z-W, Xia C-Y, Gao X-D. The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast. Fungal genetics and biology : FG & B. 2020 Nov;144:103467.
Journal cover image

Published In

Fungal genetics and biology : FG & B

DOI

EISSN

1096-0937

ISSN

1087-1845

Publication Date

November 2020

Volume

144

Start / End Page

103467

Related Subject Headings

  • rho GTP-Binding Proteins
  • Vesicular Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Microbiology
  • Membrane Proteins
  • Gene Expression Regulation, Fungal
  • Exocytosis
  • Cell Wall
  • Amino Acid Transport Systems, Basic