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The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.

Publication ,  Journal Article
Zhao, X-F; Li, M; Li, Y-Q; Chen, X-D; Gao, X-D
Published in: FEMS yeast research
February 2013

Tec1p in the budding yeast Saccharomyces cerevisiae is important for dimorphic transition. In this study, we identified a homologue of Tec1p, YlTec1p, in the distantly related dimorphic yeast Yarrowia lipolytica. YlTec1p contains an evolutionarily conserved TEA/ATTS DNA-binding domain. Expression of YlTEC1 in S. cerevisiae tec1Δ cells rescued the invasive growth defect and activated a FLO11-lacZ reporter, indicating that YlTec1p is functionally related to Tec1p. However, YlTEC1 expression failed to activate an FRE-lacZ reporter, suggesting that these two transcription factors are different. YlTEC1 plays a negative role in the yeast-to-hypha transition in Y. lipolytica based on gene deletion and overexpression studies. We show that YlTec1p activates rather than represses gene expression in Y. lipolytica by yeast one-hybrid assay, and YlTec1p is critical for the activation of FLO11-lacZ in Y. lipolytica. In addition, YlTec1p localized to the nucleus and its nuclear localization decreased during hyphal growth. We speculate that YlTec1p may normally regulate the expression of a set of target genes that may prevent rather than promote hyphal development in Y. lipolytica. Our study also suggests that YlTEC1 may not be largely regulated by the cAMP-protein kinase A pathway.

Duke Scholars

Published In

FEMS yeast research

DOI

EISSN

1567-1364

ISSN

1567-1356

Publication Date

February 2013

Volume

13

Issue

1

Start / End Page

50 / 61

Related Subject Headings

  • Yarrowia
  • Transcription Factors
  • Sequence Alignment
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Microbiology
  • Hyphae
  • Genetic Complementation Test
  • Genes, Reporter
  • Gene Expression Regulation, Fungal
 

Citation

APA
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MLA
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Zhao, X.-F., Li, M., Li, Y.-Q., Chen, X.-D., & Gao, X.-D. (2013). The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. FEMS Yeast Research, 13(1), 50–61. https://doi.org/10.1111/j.1567-1364.2012.12008.x
Zhao, Xiao-Feng, Min Li, Yun-Qing Li, Xiang-Dong Chen, and Xiang-Dong Gao. “The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.FEMS Yeast Research 13, no. 1 (February 2013): 50–61. https://doi.org/10.1111/j.1567-1364.2012.12008.x.
Zhao X-F, Li M, Li Y-Q, Chen X-D, Gao X-D. The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. FEMS yeast research. 2013 Feb;13(1):50–61.
Zhao, Xiao-Feng, et al. “The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.FEMS Yeast Research, vol. 13, no. 1, Feb. 2013, pp. 50–61. Epmc, doi:10.1111/j.1567-1364.2012.12008.x.
Zhao X-F, Li M, Li Y-Q, Chen X-D, Gao X-D. The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. FEMS yeast research. 2013 Feb;13(1):50–61.
Journal cover image

Published In

FEMS yeast research

DOI

EISSN

1567-1364

ISSN

1567-1356

Publication Date

February 2013

Volume

13

Issue

1

Start / End Page

50 / 61

Related Subject Headings

  • Yarrowia
  • Transcription Factors
  • Sequence Alignment
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Microbiology
  • Hyphae
  • Genetic Complementation Test
  • Genes, Reporter
  • Gene Expression Regulation, Fungal