Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae.
Publication
, Journal Article
Gao, X-D; Li, Y-Y
Published in: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
January 1998
The FLP-FRT site-specific recombination system in the yeast Saccharomyces cerevisiae consists of two components: FLP recombinase and FRT site. The FLP gene of the yeast 2 &mgr;m circle was placed under control of GAL10 promoter. As a result of galactose induction, the DNA sequence between two FRT direct repeats was excised by expressed FLP recombinase. Moreover, a circular plasmid containing a FRT site was site-specifically integrated into chromosome via site-specific recombination with a preset FRT site on the chromosome. The targeted DNA excision and integration events catalyzed by FLP recombinase are precise and very efficient.
Duke Scholars
Published In
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
ISSN
0582-9879
Publication Date
January 1998
Volume
30
Issue
5
Start / End Page
439 / 444
Related Subject Headings
- Biochemistry & Molecular Biology
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Gao, X.-D., & Li, Y.-Y. (1998). Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica, 30(5), 439–444.
Gao, Xiang-Dong, and Yu-Yang Li. “Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae.” Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica 30, no. 5 (January 1998): 439–44.
Gao X-D, Li Y-Y. Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 1998 Jan;30(5):439–44.
Gao, Xiang-Dong, and Yu-Yang Li. “Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae.” Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica, vol. 30, no. 5, Jan. 1998, pp. 439–44.
Gao X-D, Li Y-Y. Studies on Site-specific DNA Excision and Targeted DNA Integration in Saccharomyces cerevisiae. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 1998 Jan;30(5):439–444.
Published In
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
ISSN
0582-9879
Publication Date
January 1998
Volume
30
Issue
5
Start / End Page
439 / 444
Related Subject Headings
- Biochemistry & Molecular Biology
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology