Studies on site-specific DNA excision and targeted DNA integration in Saccharomyces cerevisiae
Publication
, Journal Article
Gao, XD; Li, YY
Published in: Acta Biochimica et Biophysica Sinica
December 1, 1998
The FLP-FRT site-specific recombination system in the yeast Saccharomyces cerevisiae consists of two components: FLP recombinase and FRT site. The FLP gene of the yeast 2 μm circle was placed under control of GAL10 promoter. As a result of galactose induction, the DNA sequence between two FRT direct repeats was excised by expressed FLP recombinase. Moreover, a circular plasmid containing a FRT site was site-specifically integrated into chromosome via site-specific recombination with a preset FRT site on the chromosome. The targeted DNA excision and integration events catalyzed by FLP recombinase are precise and very efficient.
Duke Scholars
Published In
Acta Biochimica et Biophysica Sinica
ISSN
0582-9879
Publication Date
December 1, 1998
Volume
30
Issue
5
Start / End Page
443 / 444
Related Subject Headings
- Biochemistry & Molecular Biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Gao, X. D., & Li, Y. Y. (1998). Studies on site-specific DNA excision and targeted DNA integration in Saccharomyces cerevisiae. Acta Biochimica et Biophysica Sinica, 30(5), 443–444.
Gao, X. D., and Y. Y. Li. “Studies on site-specific DNA excision and targeted DNA integration in Saccharomyces cerevisiae.” Acta Biochimica et Biophysica Sinica 30, no. 5 (December 1, 1998): 443–44.
Gao XD, Li YY. Studies on site-specific DNA excision and targeted DNA integration in Saccharomyces cerevisiae. Acta Biochimica et Biophysica Sinica. 1998 Dec 1;30(5):443–4.
Gao, X. D., and Y. Y. Li. “Studies on site-specific DNA excision and targeted DNA integration in Saccharomyces cerevisiae.” Acta Biochimica et Biophysica Sinica, vol. 30, no. 5, Dec. 1998, pp. 443–44.
Gao XD, Li YY. Studies on site-specific DNA excision and targeted DNA integration in Saccharomyces cerevisiae. Acta Biochimica et Biophysica Sinica. 1998 Dec 1;30(5):443–444.
Published In
Acta Biochimica et Biophysica Sinica
ISSN
0582-9879
Publication Date
December 1, 1998
Volume
30
Issue
5
Start / End Page
443 / 444
Related Subject Headings
- Biochemistry & Molecular Biology
- 0601 Biochemistry and Cell Biology