Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method.
Publication
, Journal Article
Young, SL; Jackson, AE; Puett, D; Melner, MH
Published in: DNA
December 1985
An assay based on high-performance liquid chromatography (HPLC) has been developed for determining the expression of the acetyl coenzyme A: chloramphenicol 3-O-acetyltransferase gene in transfected cells. The assay is based on the separation of extracted, nonradioactive chloramphenicol and its acetylated derivatives by reverse-phase HPLC, using UV absorbance for detection. In addition to eliminating the need for costly [14C]chloramphenicol, this assay is as sensitive and much faster than the commonly used method involving thin-layer chromatography.
Duke Scholars
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Published In
DNA
DOI
ISSN
0198-0238
Publication Date
December 1985
Volume
4
Issue
6
Start / End Page
469 / 475
Location
United States
Related Subject Headings
- Transfection
- Transcription, Genetic
- Genetic Markers
- Chromatography, Thin Layer
- Chromatography, High Pressure Liquid
- Chloramphenicol O-Acetyltransferase
- Cells, Cultured
- Biochemistry & Molecular Biology
- Animals
- Acetyltransferases
Citation
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Young, S. L., Jackson, A. E., Puett, D., & Melner, M. H. (1985). Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method. DNA, 4(6), 469–475. https://doi.org/10.1089/dna.1985.4.469
Young, S. L., A. E. Jackson, D. Puett, and M. H. Melner. “Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method.” DNA 4, no. 6 (December 1985): 469–75. https://doi.org/10.1089/dna.1985.4.469.
Young SL, Jackson AE, Puett D, Melner MH. Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method. DNA. 1985 Dec;4(6):469–75.
Young, S. L., et al. “Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method.” DNA, vol. 4, no. 6, Dec. 1985, pp. 469–75. Pubmed, doi:10.1089/dna.1985.4.469.
Young SL, Jackson AE, Puett D, Melner MH. Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method. DNA. 1985 Dec;4(6):469–475.
Published In
DNA
DOI
ISSN
0198-0238
Publication Date
December 1985
Volume
4
Issue
6
Start / End Page
469 / 475
Location
United States
Related Subject Headings
- Transfection
- Transcription, Genetic
- Genetic Markers
- Chromatography, Thin Layer
- Chromatography, High Pressure Liquid
- Chloramphenicol O-Acetyltransferase
- Cells, Cultured
- Biochemistry & Molecular Biology
- Animals
- Acetyltransferases