Scholars@Duke

An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins

Publication ,  Journal Article
D. Roberts, M; C. Young, K; D. Fox, C; G. Vann, C; A. Roberson, P; C. Osburn, S; H. Moore, J; W. Mumford, P; A. Romero, M; T. Beck, D ...
Published in: Journal of Biological Methods
February 24, 2020
Published version (DOI) Open Access Copy (Duke) Publisher Link

Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determining which method was most suitable for MF protein isolation and solubilization. Here, we provide an optimized protocol to isolate sarcoplasmic and solubilized MF protein fractions from mammalian skeletal muscle suitable for several downstream assays.

Duke Scholars

Chris Vann
Author Chris Vann  
Altmetric Attention Stats
Dimensions Citation Stats

Published In

Journal of Biological Methods

DOI

10.14440/jbm.2020.307

EISSN

2326-9901

Publication Date

February 24, 2020

Volume

7

Issue

1

Start / End Page

1 / 1

Publisher

Journal of Biological Methods
 

Citation

APA
Chicago
ICMJE
MLA
NLM
D. Roberts, M., C. Young, K., D. Fox, C., G. Vann, C., A. Roberson, P., C. Osburn, S., … N. Kavazis, A. (2020). An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins. Journal of Biological Methods, 7(1), 1–1. https://doi.org/10.14440/jbm.2020.307
D. Roberts, Michael, Kaelin C. Young, Carlton D. Fox, Christopher G. Vann, Paul A. Roberson, Shelby C. Osburn, Johnathon H. Moore, et al. “An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins.” Journal of Biological Methods 7, no. 1 (February 24, 2020): 1–1. https://doi.org/10.14440/jbm.2020.307.
D. Roberts M, C. Young K, D. Fox C, G. Vann C, A. Roberson P, C. Osburn S, et al. An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins. Journal of Biological Methods. 2020 Feb 24;7(1):1–1.
D. Roberts, Michael, et al. “An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins.” Journal of Biological Methods, vol. 7, no. 1, Journal of Biological Methods, Feb. 2020, pp. 1–1. Crossref, doi:10.14440/jbm.2020.307.
D. Roberts M, C. Young K, D. Fox C, G. Vann C, A. Roberson P, C. Osburn S, H. Moore J, W. Mumford P, A. Romero M, T. Beck D, T. Haun C, L.D. Badisa V, M. Mwashote B, Ibeanusi V, N. Kavazis A. An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins. Journal of Biological Methods. Journal of Biological Methods; 2020 Feb 24;7(1):1–1.

Published In

Journal of Biological Methods

DOI

10.14440/jbm.2020.307

EISSN

2326-9901

Publication Date

February 24, 2020

Volume

7

Issue

1

Start / End Page

1 / 1

Publisher

Journal of Biological Methods