Skip to main content
Journal cover image

TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii.

Publication ,  Journal Article
Hackley, RK; Hwang, S; Herb, JT; Bhanap, P; Lam, K; Vreugdenhil, A; Darnell, CL; Pastor, MM; Martin, JH; Maupin-Furlow, JA; Schmid, AK
Published in: Molecular microbiology
April 2024

Microbial cells must continually adapt their physiology in the face of changing environmental conditions. Archaea living in extreme conditions, such as saturated salinity, represent important examples of such resilience. The model salt-loving organism Haloferax volcanii exhibits remarkable plasticity in its morphology, biofilm formation, and motility in response to variations in nutrients and cell density. However, the mechanisms regulating these lifestyle transitions remain unclear. In prior research, we showed that the transcriptional regulator, TrmB, maintains the rod shape in the related species Halobacterium salinarum by activating the expression of enzyme-coding genes in the gluconeogenesis metabolic pathway. In Hbt. salinarum, TrmB-dependent production of glucose moieties is required for cell surface glycoprotein biogenesis. Here, we use a combination of genetics and quantitative phenotyping assays to demonstrate that TrmB is essential for growth under gluconeogenic conditions in Hfx. volcanii. The ∆trmB strain rapidly accumulated suppressor mutations in a gene encoding a novel transcriptional regulator, which we name trmB suppressor, or TbsP (a.k.a. "tablespoon"). TbsP is required for adhesion to abiotic surfaces (i.e., biofilm formation) and maintains wild-type cell morphology and motility. We use functional genomics and promoter fusion assays to characterize the regulons controlled by each of TrmB and TbsP, including joint regulation of the glucose-dependent transcription of gapII, which encodes an important gluconeogenic enzyme. We conclude that TrmB and TbsP coregulate gluconeogenesis, with downstream impacts on lifestyle transitions in response to nutrients in Hfx. volcanii.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Molecular microbiology

DOI

EISSN

1365-2958

ISSN

0950-382X

Publication Date

April 2024

Volume

121

Issue

4

Start / End Page

742 / 766

Related Subject Headings

  • Phenotype
  • Microbiology
  • Metabolic Networks and Pathways
  • Membrane Glycoproteins
  • Haloferax volcanii
  • Glucose
  • Archaeal Proteins
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hackley, R. K., Hwang, S., Herb, J. T., Bhanap, P., Lam, K., Vreugdenhil, A., … Schmid, A. K. (2024). TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii. Molecular Microbiology, 121(4), 742–766. https://doi.org/10.1111/mmi.15225
Hackley, Rylee K., Sungmin Hwang, Jake T. Herb, Preeti Bhanap, Katie Lam, Angie Vreugdenhil, Cynthia L. Darnell, et al. “TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii.Molecular Microbiology 121, no. 4 (April 2024): 742–66. https://doi.org/10.1111/mmi.15225.
Hackley RK, Hwang S, Herb JT, Bhanap P, Lam K, Vreugdenhil A, et al. TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii. Molecular microbiology. 2024 Apr;121(4):742–66.
Hackley, Rylee K., et al. “TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii.Molecular Microbiology, vol. 121, no. 4, Apr. 2024, pp. 742–66. Epmc, doi:10.1111/mmi.15225.
Hackley RK, Hwang S, Herb JT, Bhanap P, Lam K, Vreugdenhil A, Darnell CL, Pastor MM, Martin JH, Maupin-Furlow JA, Schmid AK. TbsP and TrmB jointly regulate gapII to influence cell development phenotypes in the archaeon Haloferax volcanii. Molecular microbiology. 2024 Apr;121(4):742–766.
Journal cover image

Published In

Molecular microbiology

DOI

EISSN

1365-2958

ISSN

0950-382X

Publication Date

April 2024

Volume

121

Issue

4

Start / End Page

742 / 766

Related Subject Headings

  • Phenotype
  • Microbiology
  • Metabolic Networks and Pathways
  • Membrane Glycoproteins
  • Haloferax volcanii
  • Glucose
  • Archaeal Proteins
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences