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Analysis of diterpenes and triterpenes from plant foliage and roots.

Publication ,  Journal Article
Wang, Q; Sohrabi, R; Tholl, D
Published in: Methods in molecular biology (Clifton, N.J.)
January 2014

Terpene specialized metabolites exhibit multiple functions in plant-environment interactions and plant development. Molecular biologists investigating the biochemistry and molecular function of terpenes need to apply robust but yet sensitive analytical methods optimized and adapted to the structural diversity and often varying concentrations of terpene compounds in plant tissues. Here we present hands-on protocols for sample preparation and GC-MS or LC-MS/MS analysis of selected diterpene and triterpene hydrocarbons or oxygenated derivatives from roots and shoots of Arabidopsis and rice.

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Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2014

Volume

1153

Start / End Page

149 / 159

Related Subject Headings

  • Triterpenes
  • Solid Phase Microextraction
  • Plant Roots
  • Plant Leaves
  • Oryza
  • Gas Chromatography-Mass Spectrometry
  • Diterpenes
  • Developmental Biology
  • Arabidopsis
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Wang, Q., Sohrabi, R., & Tholl, D. (2014). Analysis of diterpenes and triterpenes from plant foliage and roots. Methods in Molecular Biology (Clifton, N.J.), 1153, 149–159. https://doi.org/10.1007/978-1-4939-0606-2_10
Wang, Qiang, Reza Sohrabi, and Dorothea Tholl. “Analysis of diterpenes and triterpenes from plant foliage and roots.Methods in Molecular Biology (Clifton, N.J.) 1153 (January 2014): 149–59. https://doi.org/10.1007/978-1-4939-0606-2_10.
Wang Q, Sohrabi R, Tholl D. Analysis of diterpenes and triterpenes from plant foliage and roots. Methods in molecular biology (Clifton, NJ). 2014 Jan;1153:149–59.
Wang, Qiang, et al. “Analysis of diterpenes and triterpenes from plant foliage and roots.Methods in Molecular Biology (Clifton, N.J.), vol. 1153, Jan. 2014, pp. 149–59. Epmc, doi:10.1007/978-1-4939-0606-2_10.
Wang Q, Sohrabi R, Tholl D. Analysis of diterpenes and triterpenes from plant foliage and roots. Methods in molecular biology (Clifton, NJ). 2014 Jan;1153:149–159.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2014

Volume

1153

Start / End Page

149 / 159

Related Subject Headings

  • Triterpenes
  • Solid Phase Microextraction
  • Plant Roots
  • Plant Leaves
  • Oryza
  • Gas Chromatography-Mass Spectrometry
  • Diterpenes
  • Developmental Biology
  • Arabidopsis
  • 3404 Medicinal and biomolecular chemistry