Skip to main content
Journal cover image

Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown.

Publication ,  Journal Article
Atta, S; Zhao, Y; Li, JQ; Vo-Dinh, T
Published in: Analytical chemistry
March 2024

The 2019 coronavirus disease (COVID-19) outbreak created an unprecedented need for rapid, sensitive, and cost-effective point-of-care diagnostic tests to prevent and mitigate the spread of the SARS-CoV-2 virus. Herein, we demonstrated an advanced lateral flow immunoassay (LFIA) platform with dual-functional [colorimetric and surface-enhanced Raman scattering (SERS)] detection of the spike 1 (S1) protein of SARS-CoV-2. The nanosensor was integrated with a specially designed core-gap-shell morphology consisting of a gold shell decorated with external nanospheres, a structure referred to as gold nanocrown (GNC), labeled with a Raman reporter molecule 1,3,3,1',3',3'-hexamethyl-2,2'-indotricarbocyanine iodide (HITC) to produce a strong colorimetric signal as well as an enhanced SERS signal. Among the different plasmonics-active GNC nanostructures, the GNC-2 morphology, which has a shell decorated with an optimum number and size of nanospheres, produces an intense dark-blue colorimetric signal and ultrahigh SERS signal. The limit of detection (LOD) of the S1 protein via colorimetric detection LFIA was determined to be 91.24 pg/mL. On the other hand, the LOD for the SERS LFIA method was more than three orders of magnitude lower at 57.21 fg/mL. Furthermore, we analyzed the performance of the GNC-2 nanosensor for directly analyzing the S1 protein spiked in saliva samples without any sample pretreatment and achieving the LOD as low as 39.65 fg/mL using SERS-based plasmonics-enhanced LFIA, indicating ultrahigh detection sensitivity. Overall, our GNC nanosensor showed excellent sensitivity, reproducibility, and rapid detection of the SARS-CoV-2 S1 protein, demonstrating excellent potential as a promising point-of-care platform for the early detection of respiratory virus infections.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

March 2024

Volume

96

Issue

12

Start / End Page

4783 / 4790

Related Subject Headings

  • Spectrum Analysis, Raman
  • SARS-CoV-2
  • Reproducibility of Results
  • Metal Nanoparticles
  • Immunoassay
  • Humans
  • Gold
  • Colorimetry
  • COVID-19
  • Analytical Chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Atta, S., Zhao, Y., Li, J. Q., & Vo-Dinh, T. (2024). Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown. Analytical Chemistry, 96(12), 4783–4790. https://doi.org/10.1021/acs.analchem.3c04361
Atta, Supriya, Yuanhao Zhao, Joy Qiaoyi Li, and Tuan Vo-Dinh. “Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown.Analytical Chemistry 96, no. 12 (March 2024): 4783–90. https://doi.org/10.1021/acs.analchem.3c04361.
Atta, Supriya, et al. “Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown.Analytical Chemistry, vol. 96, no. 12, Mar. 2024, pp. 4783–90. Epmc, doi:10.1021/acs.analchem.3c04361.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

March 2024

Volume

96

Issue

12

Start / End Page

4783 / 4790

Related Subject Headings

  • Spectrum Analysis, Raman
  • SARS-CoV-2
  • Reproducibility of Results
  • Metal Nanoparticles
  • Immunoassay
  • Humans
  • Gold
  • Colorimetry
  • COVID-19
  • Analytical Chemistry