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Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis.

Publication ,  Journal Article
Caleffi-Ferracioli, KR; Cardoso, RF; de Souza, JV; Murase, LS; Canezin, PH; Scodro, RB; Ld Siqueira, V; Pavan, FR
Published in: Future Microbiol
February 2019
Published version (DOI) Link to item

AIM: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. MATERIALS & METHODS: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. RESULTS: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72 h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. CONCLUSION: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.

Duke Scholars

Joao Vitor Perez de Souza
Author Joao Vitor Perez de Souza Emergency Medicine

Published In

Future Microbiol

DOI

10.2217/fmb-2018-0277

EISSN

1746-0921

Publication Date

February 2019

Volume

14

Start / End Page

185 / 194

Location

England

Related Subject Headings

  • Verapamil
  • Tuberculosis, Multidrug-Resistant
  • Time Factors
  • Rifampin
  • Oxidoreductases
  • Mycobacterium tuberculosis
  • Mutation
  • Microbiology
  • Microbial Sensitivity Tests
  • Membrane Transport Proteins
 

Citation

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Chicago
ICMJE
MLA
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Caleffi-Ferracioli, K. R., Cardoso, R. F., de Souza, J. V., Murase, L. S., Canezin, P. H., Scodro, R. B., … Pavan, F. R. (2019). Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis. Future Microbiol, 14, 185–194. https://doi.org/10.2217/fmb-2018-0277
Caleffi-Ferracioli, Katiany R., Rosilene F. Cardoso, João Vp de Souza, Letícia S. Murase, Pedro H. Canezin, Regiane Bl Scodro, Vera Ld Siqueira, and Fernando R. Pavan. “Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis.” Future Microbiol 14 (February 2019): 185–94. https://doi.org/10.2217/fmb-2018-0277.
Caleffi-Ferracioli KR, Cardoso RF, de Souza JV, Murase LS, Canezin PH, Scodro RB, et al. Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis. Future Microbiol. 2019 Feb;14:185–94.
Caleffi-Ferracioli, Katiany R., et al. “Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis.” Future Microbiol, vol. 14, Feb. 2019, pp. 185–94. Pubmed, doi:10.2217/fmb-2018-0277.
Caleffi-Ferracioli KR, Cardoso RF, de Souza JV, Murase LS, Canezin PH, Scodro RB, Ld Siqueira V, Pavan FR. Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis. Future Microbiol. 2019 Feb;14:185–194.
Journal cover image

Published In

Future Microbiol

DOI

10.2217/fmb-2018-0277

EISSN

1746-0921

Publication Date

February 2019

Volume

14

Start / End Page

185 / 194

Location

England

Related Subject Headings

  • Verapamil
  • Tuberculosis, Multidrug-Resistant
  • Time Factors
  • Rifampin
  • Oxidoreductases
  • Mycobacterium tuberculosis
  • Mutation
  • Microbiology
  • Microbial Sensitivity Tests
  • Membrane Transport Proteins