Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase.
Publication
, Journal Article
Sidorenko, VS; Mechetin, GV; Nevinsky, GA; Zharkov, DO
Published in: FEBS letters
February 2008
Uracil-DNA glycosylase (Ung) can quickly locate uracil bases in an excess of undamaged DNA. DNA glycosylases may use diffusion along DNA to facilitate lesion search, resulting in processivity, the ability of glycosylases to excise closely spaced lesions without dissociating from DNA. We propose a new assay for correlated cleavage and analyze the processivity of Ung. Ung conducted correlated cleavage on double- and single-stranded substrates; the correlation declined with increasing salt concentration. Proteins in cell extracts also decreased Ung processivity. The correlated cleavage was reduced by nicks in DNA, suggesting the intact phosphodiester backbone is important for Ung processivity.
Duke Scholars
Published In
FEBS letters
DOI
EISSN
1873-3468
ISSN
0014-5793
Publication Date
February 2008
Volume
582
Issue
3
Start / End Page
410 / 414
Related Subject Headings
- Uracil-DNA Glycosidase
- Uracil
- Time Factors
- Oligonucleotides
- Molecular Sequence Data
- Kinetics
- DNA, Single-Stranded
- DNA
- Cell-Free System
- Biochemistry & Molecular Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Sidorenko, V. S., Mechetin, G. V., Nevinsky, G. A., & Zharkov, D. O. (2008). Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase. FEBS Letters, 582(3), 410–414. https://doi.org/10.1016/j.febslet.2008.01.002
Sidorenko, Viktoriya S., Grigory V. Mechetin, Georgy A. Nevinsky, and Dmitry O. Zharkov. “Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase.” FEBS Letters 582, no. 3 (February 2008): 410–14. https://doi.org/10.1016/j.febslet.2008.01.002.
Sidorenko VS, Mechetin GV, Nevinsky GA, Zharkov DO. Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase. FEBS letters. 2008 Feb;582(3):410–4.
Sidorenko, Viktoriya S., et al. “Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase.” FEBS Letters, vol. 582, no. 3, Feb. 2008, pp. 410–14. Epmc, doi:10.1016/j.febslet.2008.01.002.
Sidorenko VS, Mechetin GV, Nevinsky GA, Zharkov DO. Correlated cleavage of single- and double-stranded substrates by uracil-DNA glycosylase. FEBS letters. 2008 Feb;582(3):410–414.
Published In
FEBS letters
DOI
EISSN
1873-3468
ISSN
0014-5793
Publication Date
February 2008
Volume
582
Issue
3
Start / End Page
410 / 414
Related Subject Headings
- Uracil-DNA Glycosidase
- Uracil
- Time Factors
- Oligonucleotides
- Molecular Sequence Data
- Kinetics
- DNA, Single-Stranded
- DNA
- Cell-Free System
- Biochemistry & Molecular Biology