Skip to main content
Journal cover image

Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring.

Publication ,  Journal Article
Tissot, FLH; Cleveland, D; Grigoryan, R; Kipp, MA; Shafiee, RT; Miaou, E; Chunduri, R; Melton, H; Tacail, T; Razionale, D
Published in: Metallomics : integrated biometal science
November 2024

Calcium (Ca) isotopes in blood/urine are emerging biomarkers of bone mineral balance (BMB) in the human body. While multiple studies have investigated Ca isotopes in patients suffering from diseases affecting BMB, comparatively little effort has been devoted to understanding the homeostasis of Ca isotopes in healthy individuals. Here, we report on a longitudinal study of the urine Ca isotope composition (δ44/42CaUrine) from 22 healthy participants (age 19-60) over timescales ranging from days to months. Data from a single participant collected over a 30-day period show that morning urine is an excellent proxy for 24-h pooled urine fractions. Data from all participants reveal large inter-individual variability in δ44/42CaUrine (up to 2.2‰), which is partly due to anthropometric differences, as shown by a correlation between the participants' body mass index (BMI) and δ44/42CaUrine values. In contrast, intra-individual data reveal encouraging stability (within ∼±0.2-0.3‰) over timescales >160 days, indicating that self-referencing approaches for BMB monitoring hold greater promise than cross-sectional ones. Our data confirm that intra-individual δ44/42CaUrine variations are mainly a function of Ca reabsorption in the kidney, but also reveal the impact of other (and at times equally important) drivers, such as diet, alcohol consumption, physical exercise, or fasting. We also find that a magnetic resonance imaging contrast agent (gadolinium) can lead to artifacts during Ca isotope analysis. Based on our results, a series of practical considerations for the use of Ca isotopes in urine as tracers of BMB are presented.

Duke Scholars

Published In

Metallomics : integrated biometal science

DOI

EISSN

1756-591X

ISSN

1756-5901

Publication Date

November 2024

Volume

16

Issue

11

Start / End Page

mfae050

Related Subject Headings

  • Young Adult
  • Middle Aged
  • Male
  • Longitudinal Studies
  • Humans
  • Female
  • Calcium Isotopes
  • Calcium
  • Bone and Bones
  • Bone Density
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tissot, F. L. H., Cleveland, D., Grigoryan, R., Kipp, M. A., Shafiee, R. T., Miaou, E., … Razionale, D. (2024). Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring. Metallomics : Integrated Biometal Science, 16(11), mfae050. https://doi.org/10.1093/mtomcs/mfae050
Tissot, François L. H., Dylan Cleveland, Rosa Grigoryan, Michael A. Kipp, Roxana T. Shafiee, Emily Miaou, Rithika Chunduri, Hayward Melton, Theo Tacail, and Dan Razionale. “Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring.Metallomics : Integrated Biometal Science 16, no. 11 (November 2024): mfae050. https://doi.org/10.1093/mtomcs/mfae050.
Tissot FLH, Cleveland D, Grigoryan R, Kipp MA, Shafiee RT, Miaou E, et al. Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring. Metallomics : integrated biometal science. 2024 Nov;16(11):mfae050.
Tissot, François L. H., et al. “Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring.Metallomics : Integrated Biometal Science, vol. 16, no. 11, Nov. 2024, p. mfae050. Epmc, doi:10.1093/mtomcs/mfae050.
Tissot FLH, Cleveland D, Grigoryan R, Kipp MA, Shafiee RT, Miaou E, Chunduri R, Melton H, Tacail T, Razionale D. Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring. Metallomics : integrated biometal science. 2024 Nov;16(11):mfae050.
Journal cover image

Published In

Metallomics : integrated biometal science

DOI

EISSN

1756-591X

ISSN

1756-5901

Publication Date

November 2024

Volume

16

Issue

11

Start / End Page

mfae050

Related Subject Headings

  • Young Adult
  • Middle Aged
  • Male
  • Longitudinal Studies
  • Humans
  • Female
  • Calcium Isotopes
  • Calcium
  • Bone and Bones
  • Bone Density