Scholars@Duke publication: Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice.

Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice.

Publication , Journal Article

Han, SK; Park, SA; Jeon, JG; Chang, KW; Ahn, DK; Oh, SB; Park, SJ

Published in: Neuroscience letters

March 2009

The aim of this study was to investigate the existence of functional TRPV1 receptor by substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc), which is implicated in the processing of nociceptive information from orofacial regions. The direct membrane effects of a TRPV1 receptor agonist, capsaicin, were examined by gramicidin-perforated patch clamp recording using a trigeminal brainstem slice preparation containing Vc from immature mice. Capsaicin (1-2 microM) induced a membrane depolarization in 58 out of 71 SG neurons tested (82%). Capsaicin-induced depolarization was maintained in 20 out of 32 (63%) SG neurons in the presence of amino acid and voltage-dependent sodium channel blockers (10 microM CNQX, 20 microM AP-5, 0.5 microM TTX, 50 microM picrotoxin and 2 microM strychnine). In addition, capsaicin-induced depolarization was maintained in the presence of L-732,138 (1 microM), an NK1 receptor antagonist, in 14 out of 17 neurons (82%) tested. The capsaicin-induced depolarizing effects were blocked by a TRPV1 receptor antagonist, capsazepine (10 microM). These results indicate that a sub-population of SG neurons in the Vc express functional TRPV1 receptors, and that capsaicin can directly activate the TRPV1 receptor on the postsynaptic membrane of SG neurons.

Duke Scholars

Author Seog Oh Physics

Published In

Neuroscience letters

DOI

10.1016/j.neulet.2009.01.061

EISSN

1872-7972

ISSN

0304-3940

Publication Date

March 2009

Volume

452

Issue

Start / End Page

228 / 231

Related Subject Headings

Tryptophan
Tetrodotoxin
TRPV Cation Channels
Substantia Gelatinosa
Strychnine
Sodium Channel Blockers
Sensory System Agents
Picrotoxin
Neurons
Neurokinin-1 Receptor Antagonists

Citation

APA

Chicago

ICMJE

MLA

NLM

Han, S. K., Park, S. A., Jeon, J. G., Chang, K. W., Ahn, D. K., Oh, S. B., & Park, S. J. (2009). Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice. Neuroscience Letters, 452(3), 228–231. https://doi.org/10.1016/j.neulet.2009.01.061

Han, Seong Kyu, Seon Ah Park, Jae Gyu Jeon, Kee Wan Chang, Dong Kuk Ahn, Seok Bae Oh, and Soo Joung Park. “Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice.” Neuroscience Letters 452, no. 3 (March 2009): 228–31. https://doi.org/10.1016/j.neulet.2009.01.061.

Han SK, Park SA, Jeon JG, Chang KW, Ahn DK, Oh SB, et al. Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice. Neuroscience letters. 2009 Mar;452(3):228–31.

Han, Seong Kyu, et al. “Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice.” Neuroscience Letters, vol. 452, no. 3, Mar. 2009, pp. 228–31. Epmc, doi:10.1016/j.neulet.2009.01.061.

Han SK, Park SA, Jeon JG, Chang KW, Ahn DK, Oh SB, Park SJ. Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice. Neuroscience letters. 2009 Mar;452(3):228–231.