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A fast assay for developing serum free media for cord blood haematopoietic cells

Publication ,  Journal Article
Oh, SKW; Ng, T; See, LH
Published in: Biotechnology Techniques
August 1, 1995
Published version (DOI)

One of the bottlenecks in haematopoietic cell expansion is the lack of a defined serum free media. We describe a fast method of monitoring live haematopoietic cell numbers, using fluorescein diacetate, to facilitate the development of such a media. Several carbohydrates (fructose at 500μg/ml and pyruvate at 300μg/ml) and lipids (cholesterol and lecithin, both at 10μg/ml) were tested and could replace serum in cord blood haematopoietic cell cultures. © 1995 Chapman & Hall.

Duke Scholars

Seog Oh
Author Seog Oh Physics

Published In

Biotechnology Techniques

DOI

10.1007/BF00152447

EISSN

1573-6784

ISSN

0951-208X

Publication Date

August 1, 1995

Volume

9

Issue

8

Start / End Page

577 / 582

Related Subject Headings

  • Biotechnology
  • 40 Engineering
  • 31 Biological sciences
  • 10 Technology
  • 09 Engineering
  • 06 Biological Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Oh, S. K. W., Ng, T., & See, L. H. (1995). A fast assay for developing serum free media for cord blood haematopoietic cells. Biotechnology Techniques, 9(8), 577–582. https://doi.org/10.1007/BF00152447
Oh, S. K. W., T. Ng, and L. H. See. “A fast assay for developing serum free media for cord blood haematopoietic cells.” Biotechnology Techniques 9, no. 8 (August 1, 1995): 577–82. https://doi.org/10.1007/BF00152447.
Oh SKW, Ng T, See LH. A fast assay for developing serum free media for cord blood haematopoietic cells. Biotechnology Techniques. 1995 Aug 1;9(8):577–82.
Oh, S. K. W., et al. “A fast assay for developing serum free media for cord blood haematopoietic cells.” Biotechnology Techniques, vol. 9, no. 8, Aug. 1995, pp. 577–82. Scopus, doi:10.1007/BF00152447.
Oh SKW, Ng T, See LH. A fast assay for developing serum free media for cord blood haematopoietic cells. Biotechnology Techniques. 1995 Aug 1;9(8):577–582.

Published In

Biotechnology Techniques

DOI

10.1007/BF00152447

EISSN

1573-6784

ISSN

0951-208X

Publication Date

August 1, 1995

Volume

9

Issue

8

Start / End Page

577 / 582

Related Subject Headings

  • Biotechnology
  • 40 Engineering
  • 31 Biological sciences
  • 10 Technology
  • 09 Engineering
  • 06 Biological Sciences