Scholars@Duke publication: Long-term microcarrier suspension cultures of human embryonic stem cells.

Long-term microcarrier suspension cultures of human embryonic stem cells.

Publication , Journal Article

Oh, SKW; Chen, AK; Mok, Y; Chen, X; Lim, U-M; Chin, A; Choo, ABH; Reuveny, S

Published in: Stem cell research

May 2009

The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on matrigel-coated microcarriers achieving 2- to 4-fold higher cell densities than those in 2D colony cultures. Stable, continuous propagation of two hESC lines on microcarriers has been demonstrated in conditioned media for 6 months. Microcarrier cultures (MC) were also demonstrated in two serum-free defined media (StemPro and mTeSR1). MC achieved even higher cell concentrations in suspension spinner flasks, thus opening the prospect of propagation in controlled bioreactors.

Duke Scholars

Author Seog Oh Physics

Published In

Stem cell research

DOI

10.1016/j.scr.2009.02.005

EISSN

1876-7753

ISSN

1873-5061

Publication Date

May 2009

Volume

Issue

Start / End Page

219 / 230

Related Subject Headings

Proteoglycans
Mesoderm
Laminin
Karyotyping
Humans
Endoderm
Embryonic Stem Cells
Ectoderm
Drug Combinations
Developmental Biology

Citation

APA

Chicago

ICMJE

MLA

NLM

Oh, S. K. W., Chen, A. K., Mok, Y., Chen, X., Lim, U.-M., Chin, A., … Reuveny, S. (2009). Long-term microcarrier suspension cultures of human embryonic stem cells. Stem Cell Research, 2(3), 219–230. https://doi.org/10.1016/j.scr.2009.02.005

Oh, Steve K. W., Allen K. Chen, Yanglin Mok, Xiaoli Chen, U-Ming Lim, Angela Chin, Andre B. H. Choo, and Shaul Reuveny. “Long-term microcarrier suspension cultures of human embryonic stem cells.” Stem Cell Research 2, no. 3 (May 2009): 219–30. https://doi.org/10.1016/j.scr.2009.02.005.

Oh SKW, Chen AK, Mok Y, Chen X, Lim U-M, Chin A, et al. Long-term microcarrier suspension cultures of human embryonic stem cells. Stem cell research. 2009 May;2(3):219–30.

Oh, Steve K. W., et al. “Long-term microcarrier suspension cultures of human embryonic stem cells.” Stem Cell Research, vol. 2, no. 3, May 2009, pp. 219–30. Epmc, doi:10.1016/j.scr.2009.02.005.

Oh SKW, Chen AK, Mok Y, Chen X, Lim U-M, Chin A, Choo ABH, Reuveny S. Long-term microcarrier suspension cultures of human embryonic stem cells. Stem cell research. 2009 May;2(3):219–230.

Published In

Stem cell research

DOI

10.1016/j.scr.2009.02.005

EISSN

1876-7753

ISSN

1873-5061

Publication Date

May 2009

Volume

Issue

Start / End Page

219 / 230

Related Subject Headings

Proteoglycans
Mesoderm
Laminin
Karyotyping
Humans
Endoderm
Embryonic Stem Cells
Ectoderm
Drug Combinations
Developmental Biology