Measurement of human embryonic stem cell in the growing cycle
Publication
, Conference
Li, X; Zhao, L; Oh, SKW; Chong, WK; Ong, JK; Chen, AK; Choo, ABH
Published in: Proceedings of SPIE the International Society for Optical Engineering
December 17, 2008
A measurement and imaging system has been developed for in-line continuous measurement of live, unmodified, human embryonic stem cells (hESC). The measurement will not affect cell growth, structure, sterility and suitability for clinical use. The stem cell imaging system (SCIS) can be used to support the optimization of automated stem cell growth for in- vito study and for high-volume bio-manufacture. This paper present the experimental and analysis for the optimization of system parameters. A non-linear lighting is developed to obtain a clear images. The individual cluster can be traced from day one to day two. The whole system is calibrated with measurement microscope and haemacytometer. The measurement accuracy is better than 90%.
Duke Scholars
Published In
Proceedings of SPIE the International Society for Optical Engineering
DOI
ISSN
0277-786X
Publication Date
December 17, 2008
Volume
7155
Related Subject Headings
- 5102 Atomic, molecular and optical physics
- 4009 Electronics, sensors and digital hardware
- 4006 Communications engineering
Citation
APA
Chicago
ICMJE
MLA
NLM
Li, X., Zhao, L., Oh, S. K. W., Chong, W. K., Ong, J. K., Chen, A. K., & Choo, A. B. H. (2008). Measurement of human embryonic stem cell in the growing cycle. In Proceedings of SPIE the International Society for Optical Engineering (Vol. 7155). https://doi.org/10.1117/12.814615
Li, X., L. Zhao, S. K. W. Oh, W. K. Chong, J. K. Ong, A. K. Chen, and A. B. H. Choo. “Measurement of human embryonic stem cell in the growing cycle.” In Proceedings of SPIE the International Society for Optical Engineering, Vol. 7155, 2008. https://doi.org/10.1117/12.814615.
Li X, Zhao L, Oh SKW, Chong WK, Ong JK, Chen AK, et al. Measurement of human embryonic stem cell in the growing cycle. In: Proceedings of SPIE the International Society for Optical Engineering. 2008.
Li, X., et al. “Measurement of human embryonic stem cell in the growing cycle.” Proceedings of SPIE the International Society for Optical Engineering, vol. 7155, 2008. Scopus, doi:10.1117/12.814615.
Li X, Zhao L, Oh SKW, Chong WK, Ong JK, Chen AK, Choo ABH. Measurement of human embryonic stem cell in the growing cycle. Proceedings of SPIE the International Society for Optical Engineering. 2008.
Published In
Proceedings of SPIE the International Society for Optical Engineering
DOI
ISSN
0277-786X
Publication Date
December 17, 2008
Volume
7155
Related Subject Headings
- 5102 Atomic, molecular and optical physics
- 4009 Electronics, sensors and digital hardware
- 4006 Communications engineering