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Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.

Publication ,  Journal Article
International Stem Cell Initiative; Adewumi, O; Aflatoonian, B; Ahrlund-Richter, L; Amit, M; Andrews, PW; Beighton, G; Bello, PA; Benvenisty, N ...
Published in: Nature biotechnology
July 2007
Published version (DOI)

The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

Duke Scholars

Seog Oh
Author Seog Oh Physics

Published In

Nature biotechnology

DOI

10.1038/nbt1318

EISSN

1546-1696

ISSN

1087-0156

Publication Date

July 2007

Volume

25

Issue

7

Start / End Page

803 / 816

Related Subject Headings

  • Tetraspanin 29
  • Membrane Glycoproteins
  • Humans
  • Glycolipids
  • Genotype
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Female
  • Embryonic Stem Cells
  • Cluster Analysis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
International Stem Cell Initiative, Adewumi, O., Aflatoonian, B., Ahrlund-Richter, L., Amit, M., Andrews, P. W., … Zhang, W. (2007). Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature Biotechnology, 25(7), 803–816. https://doi.org/10.1038/nbt1318
International Stem Cell Initiative, Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W. Andrews, Gemma Beighton, et al. “Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.” Nature Biotechnology 25, no. 7 (July 2007): 803–16. https://doi.org/10.1038/nbt1318.
International Stem Cell Initiative, Adewumi O, Aflatoonian B, Ahrlund-Richter L, Amit M, Andrews PW, et al. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature biotechnology. 2007 Jul;25(7):803–16.
International Stem Cell Initiative, et al. “Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.” Nature Biotechnology, vol. 25, no. 7, July 2007, pp. 803–16. Epmc, doi:10.1038/nbt1318.
International Stem Cell Initiative, Adewumi O, Aflatoonian B, Ahrlund-Richter L, Amit M, Andrews PW, Beighton G, Bello PA, Benvenisty N, Berry LS, Bevan S, Blum B, Brooking J, Chen KG, Choo ABH, Churchill GA, Corbel M, Damjanov I, Draper JS, Dvorak P, Emanuelsson K, Fleck RA, Ford A, Gertow K, Gertsenstein M, Gokhale PJ, Hamilton RS, Hampl A, Healy LE, Hovatta O, Hyllner J, Imreh MP, Itskovitz-Eldor J, Jackson J, Johnson JL, Jones M, Kee K, King BL, Knowles BB, Lako M, Lebrin F, Mallon BS, Manning D, Mayshar Y, McKay RDG, Michalska AE, Mikkola M, Mileikovsky M, Minger SL, Moore HD, Mummery CL, Nagy A, Nakatsuji N, O’Brien CM, Oh SKW, Olsson C, Otonkoski T, Park K-Y, Passier R, Patel H, Patel M, Pedersen R, Pera MF, Piekarczyk MS, Pera RAR, Reubinoff BE, Robins AJ, Rossant J, Rugg-Gunn P, Schulz TC, Semb H, Sherrer ES, Siemen H, Stacey GN, Stojkovic M, Suemori H, Szatkiewicz J, Turetsky T, Tuuri T, van den Brink S, Vintersten K, Vuoristo S, Ward D, Weaver TA, Young LA, Zhang W. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature biotechnology. 2007 Jul;25(7):803–816.

Published In

Nature biotechnology

DOI

10.1038/nbt1318

EISSN

1546-1696

ISSN

1087-0156

Publication Date

July 2007

Volume

25

Issue

7

Start / End Page

803 / 816

Related Subject Headings

  • Tetraspanin 29
  • Membrane Glycoproteins
  • Humans
  • Glycolipids
  • Genotype
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Female
  • Embryonic Stem Cells
  • Cluster Analysis