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High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.

Publication ,  Journal Article
Drainas, AP; McIlwain, DR; Dallas, A; Chu, T; Delgado-González, A; Baron, M; Angulo-Ibáñez, M; Trejo, A; Bai, Y; Hickey, JW; Lu, G; Lu, S ...
Published in: Nature biomedical engineering
February 2025

In serology, each sample is typically tested individually, one antigen at a time. This is costly and time consuming. Serology techniques should ideally allow recurrent measurements in parallel in small sample volumes and be inexpensive and fast. Here we show that mass cytometry can be used to scale up multiplexed serology testing by leveraging polystyrene beads uniformly loaded with combinations of stable isotopes. We generated 18,480 unique isotopically barcoded beads to simultaneously detect, in a single tube with 924 serum samples, the levels of immunoglobulins G and M against 19 proteins from SARS-CoV-2 (a total of 36,960 tests in 400 nl of sample volume and 30 μl of reaction volume). As a rapid, high-throughput and cost-effective technique, serology by mass cytometry may contribute to the effective management of public health emergencies originating from infectious diseases.

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Published In

Nature biomedical engineering

DOI

EISSN

2157-846X

ISSN

2157-846X

Publication Date

February 2025

Related Subject Headings

  • 4003 Biomedical engineering
 

Citation

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Drainas, A. P., McIlwain, D. R., Dallas, A., Chu, T., Delgado-González, A., Baron, M., … Rovira-Clavé, X. (2025). High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-025-01349-0
Drainas, Alexandros P., David R. McIlwain, Alec Dallas, Theresa Chu, Antonio Delgado-González, Maya Baron, Maria Angulo-Ibáñez, et al. “High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.Nature Biomedical Engineering, February 2025. https://doi.org/10.1038/s41551-025-01349-0.
Drainas AP, McIlwain DR, Dallas A, Chu T, Delgado-González A, Baron M, et al. High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads. Nature biomedical engineering. 2025 Feb;
Drainas, Alexandros P., et al. “High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.Nature Biomedical Engineering, Feb. 2025. Epmc, doi:10.1038/s41551-025-01349-0.
Drainas AP, McIlwain DR, Dallas A, Chu T, Delgado-González A, Baron M, Angulo-Ibáñez M, Trejo A, Bai Y, Hickey JW, Lu G, Lu S, Pineda-Ramirez J, Anglin K, Richardson ET, Prostko JC, Frias E, Servellita V, Brazer N, Chiu CY, Peluso MJ, Martin JN, Wirz OF, Pham TD, Boyd SD, Kelly JD, Sage J, Nolan GP, Rovira-Clavé X. High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads. Nature biomedical engineering. 2025 Feb;

Published In

Nature biomedical engineering

DOI

EISSN

2157-846X

ISSN

2157-846X

Publication Date

February 2025

Related Subject Headings

  • 4003 Biomedical engineering