An unbiased metric of antiproliferative drug effect in vitro.
Publication
, Journal Article
Harris, LA; Frick, PL; Garbett, SP; Hardeman, KN; Paudel, BB; Lopez, CF; Quaranta, V; Tyson, DR
Published in: Nat Methods
June 2016
In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current metrics of antiproliferative small molecule effect suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.
Duke Scholars
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
June 2016
Volume
13
Issue
6
Start / End Page
497 / 500
Location
United States
Related Subject Headings
- Time Factors
- Small Molecule Libraries
- Sensitivity and Specificity
- Molecular Biology
- Models, Theoretical
- Microscopy, Fluorescence
- Humans
- Drug Discovery
- Dose-Response Relationship, Drug
- Developmental Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Harris, L. A., Frick, P. L., Garbett, S. P., Hardeman, K. N., Paudel, B. B., Lopez, C. F., … Tyson, D. R. (2016). An unbiased metric of antiproliferative drug effect in vitro. Nat Methods, 13(6), 497–500. https://doi.org/10.1038/nmeth.3852
Harris, Leonard A., Peter L. Frick, Shawn P. Garbett, Keisha N. Hardeman, B Bishal Paudel, Carlos F. Lopez, Vito Quaranta, and Darren R. Tyson. “An unbiased metric of antiproliferative drug effect in vitro.” Nat Methods 13, no. 6 (June 2016): 497–500. https://doi.org/10.1038/nmeth.3852.
Harris LA, Frick PL, Garbett SP, Hardeman KN, Paudel BB, Lopez CF, et al. An unbiased metric of antiproliferative drug effect in vitro. Nat Methods. 2016 Jun;13(6):497–500.
Harris, Leonard A., et al. “An unbiased metric of antiproliferative drug effect in vitro.” Nat Methods, vol. 13, no. 6, June 2016, pp. 497–500. Pubmed, doi:10.1038/nmeth.3852.
Harris LA, Frick PL, Garbett SP, Hardeman KN, Paudel BB, Lopez CF, Quaranta V, Tyson DR. An unbiased metric of antiproliferative drug effect in vitro. Nat Methods. 2016 Jun;13(6):497–500.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
June 2016
Volume
13
Issue
6
Start / End Page
497 / 500
Location
United States
Related Subject Headings
- Time Factors
- Small Molecule Libraries
- Sensitivity and Specificity
- Molecular Biology
- Models, Theoretical
- Microscopy, Fluorescence
- Humans
- Drug Discovery
- Dose-Response Relationship, Drug
- Developmental Biology