A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis

Lyme disease, the most common vector-borne illness in North America, is caused by the spirochete. Infection begins in the skin following a tick bite and can spread to the hearts, joints, nervous system, and other organs. Diverse host responses influence the level of.infection in mice and humans. Using a systems biology approach, we examined potential molecular interactions between human extracellular and secreted proteins and.. A yeast display library expressing 1031 human extracellular proteins was probed against 36 isolates of.. We found that human Peptidoglycan Recognition Protein 1 (PGLYRP1) interacted with the vast majority of.isolates. In subsequent experiments, we demonstrated that recombinant PGLYRP1 interacts with purified.peptidoglycan and exhibits borreliacidal activity, suggesting that vertebrate hosts may use PGLYRP1 to identify.. We examined.infection in mice lacking PGLYRP1 and observed an increased spirochete burden in the heart and joints, along with splenomegaly. Mice lacking PGLYRP1 also showed signs of immune dysregulation, including lower serum IgG levels and higher levels of IFNγ, CXCL9, and CXCL10.Taken together, our findings suggest that PGLYRP1 plays a role in the host’s response to.and further demonstrate the utility of expansive yeast display screening in capturing biologically relevant interactions between spirochetes and their hosts.

Duke Scholars

Author Akash Kishore Gupta Medicine, Rheumatology and Immunology