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Metagenomics Bioinformatic Pipeline.

Publication ,  Chapter
Garfias-Gallegos, D; Zirión-Martínez, C; Bustos-Díaz, ED; Arellano-Fernández, TV; Lovaco-Flores, JA; Espinosa-Jaime, A; Avelar-Rivas, JA ...
January 2022

Microbial communities' taxonomic and functional diversity has been broadly studied since sequencing technologies enabled faster and cheaper data obtainment. Nevertheless, the programming skills needed and the amount of software available may be overwhelming to someone trying to analyze these data. Here, we present a comprehensive and straightforward pipeline that takes shotgun metagenomics data through the needed steps to obtain valuable results. The raw data goes through a quality control process, metagenomic assembly, binning (the obtention of single genomes from a metagenome), taxonomic assignment, and taxonomic diversity analysis and visualization.

Duke Scholars

DOI

Publication Date

January 2022

Volume

2512

Start / End Page

153 / 179

Related Subject Headings

  • Software
  • Sequence Analysis, DNA
  • Microbiota
  • Metagenomics
  • Metagenome
  • Developmental Biology
  • Computational Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Chicago
ICMJE
MLA
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Garfias-Gallegos, D., Zirión-Martínez, C., Bustos-Díaz, E. D., Arellano-Fernández, T. V., Lovaco-Flores, J. A., Espinosa-Jaime, A., … Sélem-Mójica, N. (2022). Metagenomics Bioinformatic Pipeline. (Vol. 2512, pp. 153–179). https://doi.org/10.1007/978-1-0716-2429-6_10
Garfias-Gallegos, Diego, Claudia Zirión-Martínez, Edder D. Bustos-Díaz, Tania Vanessa Arellano-Fernández, José Abel Lovaco-Flores, Aarón Espinosa-Jaime, J Abraham Avelar-Rivas, and Nelly Sélem-Mójica. “Metagenomics Bioinformatic Pipeline.,” 2512:153–79, 2022. https://doi.org/10.1007/978-1-0716-2429-6_10.
Garfias-Gallegos D, Zirión-Martínez C, Bustos-Díaz ED, Arellano-Fernández TV, Lovaco-Flores JA, Espinosa-Jaime A, et al. Metagenomics Bioinformatic Pipeline. In 2022. p. 153–79.
Garfias-Gallegos, Diego, et al. Metagenomics Bioinformatic Pipeline. Vol. 2512, 2022, pp. 153–79. Epmc, doi:10.1007/978-1-0716-2429-6_10.
Garfias-Gallegos D, Zirión-Martínez C, Bustos-Díaz ED, Arellano-Fernández TV, Lovaco-Flores JA, Espinosa-Jaime A, Avelar-Rivas JA, Sélem-Mójica N. Metagenomics Bioinformatic Pipeline. 2022. p. 153–179.

DOI

Publication Date

January 2022

Volume

2512

Start / End Page

153 / 179

Related Subject Headings

  • Software
  • Sequence Analysis, DNA
  • Microbiota
  • Metagenomics
  • Metagenome
  • Developmental Biology
  • Computational Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology