Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients.
Publication
, Journal Article
Mah, JK; Hogan, JI; Kothadia, S; Keenan, JE; Berger, J; Carugati, M
Published in: Med Mycol Case Rep
December 2025
A cluster of Aspergillus fumigatus donor-derived infections (DDI) was rapidly diagnosed using plasma metagenomic next-generation sequencing (mNGS) among solid organ transplant recipients. The heart recipient, experiencing marginal hemodynamics, underwent an endomyocardial biopsy, which was concerning for a fungal infection on histopathology. Plasma mNGS was performed, identifying A. fumigatus two days prior to conventional diagnostics. This timely diagnosis enabled prompt nephrectomies in the kidney recipients, who survived. This report represents the first published use of mNGS in the diagnosis of Aspergillus fumigatus DDI, highlighting the utility of this novel, underutilized assay for early diagnosis of donor-derived infections.
Duke Scholars
Published In
Med Mycol Case Rep
DOI
ISSN
2211-7539
Publication Date
December 2025
Volume
50
Start / End Page
100751
Location
Netherlands
Related Subject Headings
- 3207 Medical microbiology
Citation
APA
Chicago
ICMJE
MLA
NLM
Mah, J. K., Hogan, J. I., Kothadia, S., Keenan, J. E., Berger, J., & Carugati, M. (2025). Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients. Med Mycol Case Rep, 50, 100751. https://doi.org/10.1016/j.mmcr.2025.100751
Mah, Jordan K., John I. Hogan, Sonya Kothadia, Jeffrey E. Keenan, Johnathan Berger, and Manuela Carugati. “Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients.” Med Mycol Case Rep 50 (December 2025): 100751. https://doi.org/10.1016/j.mmcr.2025.100751.
Mah JK, Hogan JI, Kothadia S, Keenan JE, Berger J, Carugati M. Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients. Med Mycol Case Rep. 2025 Dec;50:100751.
Mah, Jordan K., et al. “Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients.” Med Mycol Case Rep, vol. 50, Dec. 2025, p. 100751. Pubmed, doi:10.1016/j.mmcr.2025.100751.
Mah JK, Hogan JI, Kothadia S, Keenan JE, Berger J, Carugati M. Application of plasma cell-free metagenomic next-generation sequencing for the identification of Aspergillus fumigatus donor-derived infections among solid organ transplant recipients. Med Mycol Case Rep. 2025 Dec;50:100751.
Published In
Med Mycol Case Rep
DOI
ISSN
2211-7539
Publication Date
December 2025
Volume
50
Start / End Page
100751
Location
Netherlands
Related Subject Headings
- 3207 Medical microbiology