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Assessment of purging with multidrug resistance (MDR) modulators and VP-16: results of long-term marrow culture.

Publication ,  Journal Article
Aihara, M; Sikic, BI; Blume, KG; Chao, NJ
Published in: Exp Hematol
September 1990

We studied the effects of two modulators of multidrug resistance (MDR), cyclosporine and verapamil, on the cytotoxicity of etoposide (VP-16) in normal bone marrow cells. VP-16 was toxic to normal bone marrow at concentrations greater than 50 microM, resulting in no granulocyte-macrophage colony-forming units (CFU-GM) in short-term methylcellulose cultures. However, in long-term marrow cultures (LTMC) treatment with VP-16 without the addition of MDR modulators resulted in only a twofold decrease in total cell number at a VP-16 concentration of 50 microM, compared to media alone in the adherent cell layer, and approximately 20% recovery of CFU-GM. The addition of MDR modulators did not result in excessive cytotoxicity, reducing the total CFU-GM by two- to threefold even at the higher VP-16 concentration. Therefore, these modulators in conjunction with VP-16 can be safely used on normal bone marrow cells and may provide an effective method to purge MDR-tumor cells.

Duke Scholars

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

September 1990

Volume

18

Issue

8

Start / End Page

940 / 944

Location

Netherlands

Related Subject Headings

  • Verapamil
  • Methylcellulose
  • Macrophages
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Granulocytes
  • Etoposide
  • Drug Resistance
  • Cyclosporins
 

Citation

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Aihara, M., Sikic, B. I., Blume, K. G., & Chao, N. J. (1990). Assessment of purging with multidrug resistance (MDR) modulators and VP-16: results of long-term marrow culture. Exp Hematol, 18(8), 940–944.
Aihara, M., B. I. Sikic, K. G. Blume, and N. J. Chao. “Assessment of purging with multidrug resistance (MDR) modulators and VP-16: results of long-term marrow culture.Exp Hematol 18, no. 8 (September 1990): 940–44.
Aihara M, Sikic BI, Blume KG, Chao NJ. Assessment of purging with multidrug resistance (MDR) modulators and VP-16: results of long-term marrow culture. Exp Hematol. 1990 Sep;18(8):940–4.
Aihara, M., et al. “Assessment of purging with multidrug resistance (MDR) modulators and VP-16: results of long-term marrow culture.Exp Hematol, vol. 18, no. 8, Sept. 1990, pp. 940–44.
Aihara M, Sikic BI, Blume KG, Chao NJ. Assessment of purging with multidrug resistance (MDR) modulators and VP-16: results of long-term marrow culture. Exp Hematol. 1990 Sep;18(8):940–944.
Journal cover image

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

September 1990

Volume

18

Issue

8

Start / End Page

940 / 944

Location

Netherlands

Related Subject Headings

  • Verapamil
  • Methylcellulose
  • Macrophages
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Granulocytes
  • Etoposide
  • Drug Resistance
  • Cyclosporins