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Prevention of graft-versus-host disease by peptides binding to class II major histocompatibility complex molecules.

Publication ,  Journal Article
Schlegel, PG; Aharoni, R; Smilek, DE; Fernandez, LP; McDevitt, HO; Tran, N; Vaysburd, M; Chao, NJ
Published in: Blood
October 15, 1994

Graft-versus-host disease across minor histocompatibility barriers was induced in two different models by transplanting allogeneic bone marrow and spleen cells into irradiated H-2-compatible recipient mice. In this report, we show that administration of peptides with high binding affinity for the respective class II major histocompatibility complex molecules after transplantation is capable of preventing the development of graft-versus-host disease in two different murine models. The peptides used were myelin basic protein residues 1 through 11 with alanine at position 4 (Ac 1-11[4A]) for I-Au (A alpha uA beta u), and the antigenic core sequence 323 through 339 of ovalbumin with lysine and methionine extension (KM core) for I-As (A alpha sA beta s). In both systems, the mechanism of prevention was found to be major histocompatibility complex-associated, because nonbinding control peptides did not have any effect. Engraftment of allogeneic bone marrow cells was shown by polymerase chain reaction analysis of DNA polymorphisms in a microsatellite region within the murine interleukin-5 gene.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

October 15, 1994

Volume

84

Issue

8

Start / End Page

2802 / 2810

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Repetitive Sequences, Nucleic Acid
  • Polymerase Chain Reaction
  • Peptide Fragments
  • Ovalbumin
  • Myelin Basic Protein
  • Molecular Sequence Data
  • Mice
  • Interleukin-5
 

Citation

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MLA
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Schlegel, P. G., Aharoni, R., Smilek, D. E., Fernandez, L. P., McDevitt, H. O., Tran, N., … Chao, N. J. (1994). Prevention of graft-versus-host disease by peptides binding to class II major histocompatibility complex molecules. Blood, 84(8), 2802–2810.
Schlegel, P. G., R. Aharoni, D. E. Smilek, L. P. Fernandez, H. O. McDevitt, N. Tran, M. Vaysburd, and N. J. Chao. “Prevention of graft-versus-host disease by peptides binding to class II major histocompatibility complex molecules.Blood 84, no. 8 (October 15, 1994): 2802–10.
Schlegel PG, Aharoni R, Smilek DE, Fernandez LP, McDevitt HO, Tran N, et al. Prevention of graft-versus-host disease by peptides binding to class II major histocompatibility complex molecules. Blood. 1994 Oct 15;84(8):2802–10.
Schlegel, P. G., et al. “Prevention of graft-versus-host disease by peptides binding to class II major histocompatibility complex molecules.Blood, vol. 84, no. 8, Oct. 1994, pp. 2802–10.
Schlegel PG, Aharoni R, Smilek DE, Fernandez LP, McDevitt HO, Tran N, Vaysburd M, Chao NJ. Prevention of graft-versus-host disease by peptides binding to class II major histocompatibility complex molecules. Blood. 1994 Oct 15;84(8):2802–2810.

Published In

Blood

ISSN

0006-4971

Publication Date

October 15, 1994

Volume

84

Issue

8

Start / End Page

2802 / 2810

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Repetitive Sequences, Nucleic Acid
  • Polymerase Chain Reaction
  • Peptide Fragments
  • Ovalbumin
  • Myelin Basic Protein
  • Molecular Sequence Data
  • Mice
  • Interleukin-5