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Does thalidomide affect IL-2 response and production?

Publication ,  Journal Article
Fernandez, LP; Schlegel, PG; Baker, J; Chen, Y; Chao, NJ
Published in: Exp Hematol
August 1995

The exact mechanism of immunosuppression by thalidomide is poorly understood. A common denominator in the pathogenesis of graft-vs.-host disease, graft rejection, reactional lepromatous leprosy, and autoimmune disorders modulated by thalidomide is the activation of T lymphocytes culminating in the synthesis of interleukin-2 (IL-2), the expression of high-affinity IL-2 receptors, and the induction of proliferation. We investigated the effect of thalidomide on the production of IL-2 by the human leukemia cell line Jurkat through induction of IL-2 gene enhancer activity and through the presence of IL-2 in supernatants. beta-galactosidase activity, encoded by a reporter lac z construct and controlled by a transcription factor in thalidomide-treated PMA- and ionomycin-stimulated Jurkat cells, was similar (97 +/- 1.33%; p > 0.1) to non-thalidomide-treated controls at all drug concentrations tested. IL-2 enhancer-driven beta-galactose activity of thalidomide-treated and stimulated cells was also similar to that of untreated controls (p > 0.2). The IL-2 production of activated nontransfected Jurkat cells was gauged by using the IL-2-dependent cell line HT-2 as a readout and by ELISA. Jurkat cells were subcloned by limiting dilution. Bulk cultures and three subclones (J.5.2.5., J.5.2.9., and J.5.3.8.) were assayed at 6, 12, and 24 hours after PHA/PMA-induced stimulation. No inhibitory effect on the IL-2 production by thalidomide could be detected at any of the drug concentrations tested (5-30 micrograms/mL), whereas 10 to 100 ng/mL of cyclosporine inhibited the IL-2 production by 95 to 100%. In addition, we observed neither inhibition of IL-2-dependent proliferation of HT-2 nor inhibition of PHA-induced proliferation of peripheral mononuclear cells by thalidomide at all drug concentrations used (5-30 micrograms/mL). These results do not support the possibility of a modulatory effect on the immune response by thalidomide via IL-2 production and IL-2 response.

Duke Scholars

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

August 1995

Volume

23

Issue

9

Start / End Page

978 / 985

Location

Netherlands

Related Subject Headings

  • beta-Galactosidase
  • Tumor Cells, Cultured
  • Transfection
  • Thalidomide
  • Tetradecanoylphorbol Acetate
  • Lymphocytes
  • Lymphocyte Activation
  • Leukemia
  • Kinetics
  • Ionomycin
 

Citation

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Chicago
ICMJE
MLA
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Fernandez, L. P., Schlegel, P. G., Baker, J., Chen, Y., & Chao, N. J. (1995). Does thalidomide affect IL-2 response and production? Exp Hematol, 23(9), 978–985.
Fernandez, L. P., P. G. Schlegel, J. Baker, Y. Chen, and N. J. Chao. “Does thalidomide affect IL-2 response and production?Exp Hematol 23, no. 9 (August 1995): 978–85.
Fernandez LP, Schlegel PG, Baker J, Chen Y, Chao NJ. Does thalidomide affect IL-2 response and production? Exp Hematol. 1995 Aug;23(9):978–85.
Fernandez, L. P., et al. “Does thalidomide affect IL-2 response and production?Exp Hematol, vol. 23, no. 9, Aug. 1995, pp. 978–85.
Fernandez LP, Schlegel PG, Baker J, Chen Y, Chao NJ. Does thalidomide affect IL-2 response and production? Exp Hematol. 1995 Aug;23(9):978–985.
Journal cover image

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

August 1995

Volume

23

Issue

9

Start / End Page

978 / 985

Location

Netherlands

Related Subject Headings

  • beta-Galactosidase
  • Tumor Cells, Cultured
  • Transfection
  • Thalidomide
  • Tetradecanoylphorbol Acetate
  • Lymphocytes
  • Lymphocyte Activation
  • Leukemia
  • Kinetics
  • Ionomycin