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Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion.

Publication ,  Journal Article
Harrison, JK; Fong, AM; Swain, PA; Chen, S; Yu, YR; Salafranca, MN; Greenleaf, WB; Imai, T; Patel, DD
Published in: J Biol Chem
June 15, 2001

Fractalkine (FKN/CX3CL1) is a unique member of the chemokine gene family and contains a chemokine domain (CD), a mucin-like stalk, a single transmembrane region, and a short intracellular C terminus. This structural distinction affords FKN the property of mediating capture and firm adhesion of FKN receptor (CX3CR1)-expressing cells under physiological flow conditions. Shed forms of FKN also exist, and these promote chemotaxis of CX3CR1-expressing leukocytes. The goal of the present study was to identify specific residues within the FKN-CD critical for FKN-CX3CR1 interactions. Two residues were identified in the FKN-CD, namely Lys-7 and Arg-47, that are important determinants in mediating an FKN-CX3CR1 interaction. FKN-K7A and FKN-R47A mutants exhibited 30-60-fold decreases in affinity for CX3CR1 and failed to arrest efficiently CX3CR1-expressing cells under physiological flow conditions. However, these mutants had differential effects on chemotaxis of CX3CR1-expressing cells. The FKN-K7A mutant acted as an equipotent partial agonist, whereas the FKN-R47A mutant had marked decreased potency and efficacy in measures of chemotactic activity. These data identify specific structural features of the FKN-CD that are important in interactions with CX3CR1 including steady state binding, signaling, and firm adhesion of CX3CR1-expressing cells.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

June 15, 2001

Volume

276

Issue

24

Start / End Page

21632 / 21641

Location

United States

Related Subject Headings

  • Transfection
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Recombinant Proteins
  • Receptors, HIV
  • Receptors, Cytokine
  • Rats
  • Protein Structure, Secondary
  • Polymerase Chain Reaction
 

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Harrison, J. K., Fong, A. M., Swain, P. A., Chen, S., Yu, Y. R., Salafranca, M. N., … Patel, D. D. (2001). Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion. J Biol Chem, 276(24), 21632–21641. https://doi.org/10.1074/jbc.M010261200
Harrison, J. K., A. M. Fong, P. A. Swain, S. Chen, Y. R. Yu, M. N. Salafranca, W. B. Greenleaf, T. Imai, and D. D. Patel. “Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion.J Biol Chem 276, no. 24 (June 15, 2001): 21632–41. https://doi.org/10.1074/jbc.M010261200.
Harrison JK, Fong AM, Swain PA, Chen S, Yu YR, Salafranca MN, et al. Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion. J Biol Chem. 2001 Jun 15;276(24):21632–41.
Harrison, J. K., et al. “Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion.J Biol Chem, vol. 276, no. 24, June 2001, pp. 21632–41. Pubmed, doi:10.1074/jbc.M010261200.
Harrison JK, Fong AM, Swain PA, Chen S, Yu YR, Salafranca MN, Greenleaf WB, Imai T, Patel DD. Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion. J Biol Chem. 2001 Jun 15;276(24):21632–21641.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

June 15, 2001

Volume

276

Issue

24

Start / End Page

21632 / 21641

Location

United States

Related Subject Headings

  • Transfection
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Recombinant Proteins
  • Receptors, HIV
  • Receptors, Cytokine
  • Rats
  • Protein Structure, Secondary
  • Polymerase Chain Reaction