Skip to main content
Journal cover image

N-terminal amino acid sequences of three functionally different troponin T isoforms from rabbit fast skeletal muscle.

Publication ,  Journal Article
Briggs, MM; Schachat, F
Published in: J Mol Biol
March 5, 1989

The different isoforms of fast skeletal muscle troponin T (TnT) are generated by alternative splicing of several 5' exons in the fast TnT gene. In rabbit skeletal muscle this process results in three major fast TnT species, TnT1f, TnT2f and TnT3f, that differ in a region of 30 to 40 amino acid residues near the N terminus. Differential expression of these three isoforms modulates the activation of the thin filament by calcium. To establish a basis for further structure-function studies, we have sequenced the N-terminal region of these proteins. TnT2f is the fast TnT sequenced by Pearlstone et al. The larger species TnT1f contains six additional amino acid residues identical in sequence and position to those encoded by exon 4 in the rat fast skeletal muscle TnT gene. TnT3f also contains that sequence but lacks 17 amino acid residues spanning the region encoded by exons 6 and 7 of the rat gene. These three TnTs appear to be generated by discrete alternative splicing pathways, each differing by a single event. Comparison of these TnT sequences with those from chicken fast skeletal muscle and bovine heart shows that the splicing pattern resulting in the excision of exon 4 is evolutionarily conserved and leads to a more calcium-sensitive thin filament.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

March 5, 1989

Volume

206

Issue

1

Start / End Page

245 / 249

Location

Netherlands

Related Subject Headings

  • Troponin T
  • Troponin
  • Rabbits
  • Muscles
  • Molecular Sequence Data
  • Biochemistry & Molecular Biology
  • Animals
  • Amino Acids
  • Amino Acid Sequence
  • 3107 Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Briggs, M. M., & Schachat, F. (1989). N-terminal amino acid sequences of three functionally different troponin T isoforms from rabbit fast skeletal muscle. J Mol Biol, 206(1), 245–249. https://doi.org/10.1016/0022-2836(89)90538-x
Briggs, M. M., and F. Schachat. “N-terminal amino acid sequences of three functionally different troponin T isoforms from rabbit fast skeletal muscle.J Mol Biol 206, no. 1 (March 5, 1989): 245–49. https://doi.org/10.1016/0022-2836(89)90538-x.
Briggs, M. M., and F. Schachat. “N-terminal amino acid sequences of three functionally different troponin T isoforms from rabbit fast skeletal muscle.J Mol Biol, vol. 206, no. 1, Mar. 1989, pp. 245–49. Pubmed, doi:10.1016/0022-2836(89)90538-x.
Journal cover image

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

March 5, 1989

Volume

206

Issue

1

Start / End Page

245 / 249

Location

Netherlands

Related Subject Headings

  • Troponin T
  • Troponin
  • Rabbits
  • Muscles
  • Molecular Sequence Data
  • Biochemistry & Molecular Biology
  • Animals
  • Amino Acids
  • Amino Acid Sequence
  • 3107 Microbiology