Scholars@Duke publication: Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes.

Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes.

Publication , Journal Article

Pinto-Correia, C; Goldstein, EG; Bennett, V; Sobel, JS

Published in: Dev Biol

August 1991

The mouse oocyte expresses a polypeptide of Mr 120,000 that cross-reacts with an antibody to the brain membrane skeletal protein adducin. Immunofluorescence localization showed a bright chromosomal staining reaction in metaphase I and metaphase II oocytes. Following in vitro fertilization the maternal chromosomes lost their immunoreactivity during pronuclear development. The fertilizing sperm chromatin and male pronucleus did not show any detectable staining reaction. Bright chromosomal fluorescence was again observed in the first mitotic metaphase when both maternal and paternal chromosomes gave a positive staining reaction. In contrast to the immunoreactivity of the maternal meiotic chromosomes, the meiotic chromosomes of male germ line cells failed to exhibit any detectable staining reaction and this difference was confirmed by immunolabeling of oocyte and spermatocyte karyotypes. Mitotic chromosomes in preimplantation embryos, fetal liver, adult intestinal epithelium, and MDCK cells also failed to show any detectable labeling reaction. The results suggest that expression of the immunoreactive chromosomal adducin may be a unique feature of oogenesis.

Duke Scholars

Author Vann Bennett Biochemistry

Published In

Dev Biol

DOI

10.1016/0012-1606(91)90232-r

ISSN

0012-1606

Publication Date

August 1991

Volume

146

Issue

Start / End Page

301 / 311

Location

United States

Related Subject Headings

Testis
Oocytes
Molecular Weight
Mice, Inbred ICR
Mice
Male
Intestines
Fluorescent Antibody Technique
Fetus
Female

Citation

APA

Chicago

ICMJE

MLA

NLM

Pinto-Correia, C., Goldstein, E. G., Bennett, V., & Sobel, J. S. (1991). Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes. Dev Biol, 146(2), 301–311. https://doi.org/10.1016/0012-1606(91)90232-r

Pinto-Correia, C., E. G. Goldstein, V. Bennett, and J. S. Sobel. “Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes.” Dev Biol 146, no. 2 (August 1991): 301–11. https://doi.org/10.1016/0012-1606(91)90232-r.

Pinto-Correia C, Goldstein EG, Bennett V, Sobel JS. Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes. Dev Biol. 1991 Aug;146(2):301–11.

Pinto-Correia, C., et al. “Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes.” Dev Biol, vol. 146, no. 2, Aug. 1991, pp. 301–11. Pubmed, doi:10.1016/0012-1606(91)90232-r.

Pinto-Correia C, Goldstein EG, Bennett V, Sobel JS. Immunofluorescence localization of an adducin-like protein in the chromosomes of mouse oocytes. Dev Biol. 1991 Aug;146(2):301–311.