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Tyrosine phosphorylation of G protein alpha subunits by pp60c-src.

Publication ,  Journal Article
Hausdorff, WP; Pitcher, JA; Luttrell, DK; Linder, ME; Kurose, H; Parsons, SJ; Caron, MG; Lefkowitz, RJ
Published in: Proc Natl Acad Sci U S A
July 1, 1992
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A number of lines of evidence suggest that cross-talk exists between the cellular signal transduction pathways involving tyrosine phosphorylation catalyzed by members of the pp60c-src kinase family and those mediated by guanine nucleotide regulatory proteins (G proteins). In this study, we explore the possibility that direct interactions between pp60c-src and G proteins may occur with functional consequences. Preparations of pp60c-src isolated by immunoprecipitation phosphorylate on tyrosine residues the purified G-protein alpha subunits (G alpha) of several heterotrimeric G proteins. Phosphorylation is highly dependent on G-protein conformation, and G alpha(GDP) uncomplexed by beta gamma subunits appears to be the preferred substrate. In functional studies, phosphorylation of stimulatory G alpha (G alpha s) modestly increases the rate of binding of guanosine 5'-[gamma-[35S]thio]triphosphate to Gs as well as the receptor-stimulated steady-state rate of GTP hydrolysis by Gs. Heterotrimeric G proteins may represent a previously unappreciated class of potential substrates for pp60c-src.

Duke Scholars

Robert J. Lefkowitz
Author Robert J. Lefkowitz Medicine, Cardiology
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.89.13.5720

ISSN

0027-8424

Publication Date

July 1, 1992

Volume

89

Issue

13

Start / End Page

5720 / 5724

Location

United States

Related Subject Headings

  • Tyrosine
  • Structure-Activity Relationship
  • Signal Transduction
  • Proto-Oncogene Proteins pp60(c-src)
  • Protein Conformation
  • Phosphotyrosine
  • Phosphorylation
  • Phosphoproteins
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Guanine Nucleotides
 

Citation

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Hausdorff, W. P., Pitcher, J. A., Luttrell, D. K., Linder, M. E., Kurose, H., Parsons, S. J., … Lefkowitz, R. J. (1992). Tyrosine phosphorylation of G protein alpha subunits by pp60c-src. Proc Natl Acad Sci U S A, 89(13), 5720–5724. https://doi.org/10.1073/pnas.89.13.5720
Hausdorff, W. P., J. A. Pitcher, D. K. Luttrell, M. E. Linder, H. Kurose, S. J. Parsons, M. G. Caron, and R. J. Lefkowitz. “Tyrosine phosphorylation of G protein alpha subunits by pp60c-src.” Proc Natl Acad Sci U S A 89, no. 13 (July 1, 1992): 5720–24. https://doi.org/10.1073/pnas.89.13.5720.
Hausdorff WP, Pitcher JA, Luttrell DK, Linder ME, Kurose H, Parsons SJ, et al. Tyrosine phosphorylation of G protein alpha subunits by pp60c-src. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5720–4.
Hausdorff, W. P., et al. “Tyrosine phosphorylation of G protein alpha subunits by pp60c-src.” Proc Natl Acad Sci U S A, vol. 89, no. 13, July 1992, pp. 5720–24. Pubmed, doi:10.1073/pnas.89.13.5720.
Hausdorff WP, Pitcher JA, Luttrell DK, Linder ME, Kurose H, Parsons SJ, Caron MG, Lefkowitz RJ. Tyrosine phosphorylation of G protein alpha subunits by pp60c-src. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5720–5724.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.89.13.5720

ISSN

0027-8424

Publication Date

July 1, 1992

Volume

89

Issue

13

Start / End Page

5720 / 5724

Location

United States

Related Subject Headings

  • Tyrosine
  • Structure-Activity Relationship
  • Signal Transduction
  • Proto-Oncogene Proteins pp60(c-src)
  • Protein Conformation
  • Phosphotyrosine
  • Phosphorylation
  • Phosphoproteins
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Guanine Nucleotides