Scholars@Duke publication: Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone.

Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone.

Publication , Journal Article

Collins, S; Quarmby, VE; French, FS; Lefkowitz, RJ; Caron, MG

Published in: FEBS Lett

June 6, 1988

To investigate the regulation of beta 2AR expression in rat ventral prostate the effects of castration and testosterone replacement on the beta 2AR were studied by ligand binding and Northern blot analysis. Orchidectomy depressed beta 2AR number by 50% within 4 days and testosterone administration to 4-day castrates produced a rapid and complete recovery of beta 2AR number within 24 h. In contrast to receptor number, beta 2AR mRNA levels did not change relative to total RNA following castration. However, during the testosterone replacement period beta 2AR mRNA levels rose transiently, reaching a maximum (3.5-fold) between 8 and 12 h, and this increase in mRNA preceded the recovery in beta 2AR number in the membrane. Regulation of beta 2AR gene expression by testosterone in the ventral prostate is thus complex and probably involves both transcriptional and post-transcriptional components.

Duke Scholars

Author Robert J. Lefkowitz Medicine, Cardiology

Published In

FEBS Lett

DOI

10.1016/0014-5793(88)81378-4

ISSN

0014-5793

Publication Date

June 6, 1988

Volume

233

Issue

Start / End Page

173 / 176

Location

England

Related Subject Headings

Transcription, Genetic
Testosterone
Receptors, Adrenergic, beta
Rats, Inbred Strains
Rats
RNA, Messenger
Prostate
Pindolol
Orchiectomy
Nucleic Acid Hybridization

Citation

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Chicago

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MLA

NLM

Collins, S., Quarmby, V. E., French, F. S., Lefkowitz, R. J., & Caron, M. G. (1988). Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone. FEBS Lett, 233(1), 173–176. https://doi.org/10.1016/0014-5793(88)81378-4

Collins, S., V. E. Quarmby, F. S. French, R. J. Lefkowitz, and M. G. Caron. “Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone.” FEBS Lett 233, no. 1 (June 6, 1988): 173–76. https://doi.org/10.1016/0014-5793(88)81378-4.

Collins S, Quarmby VE, French FS, Lefkowitz RJ, Caron MG. Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone. FEBS Lett. 1988 Jun 6;233(1):173–6.

Collins, S., et al. “Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone.” FEBS Lett, vol. 233, no. 1, June 1988, pp. 173–76. Pubmed, doi:10.1016/0014-5793(88)81378-4.

Collins S, Quarmby VE, French FS, Lefkowitz RJ, Caron MG. Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone. FEBS Lett. 1988 Jun 6;233(1):173–176.