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Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme.

Publication ,  Journal Article
Perrotta, AT; Shih, I; Been, MD
Published in: Science
October 1, 1999

Ribozymes use a number of the same catalytic strategies as protein enzymes. However, general base catalysis by a ribozyme has not been demonstrated. In the hepatitis delta virus antigenomic ribozyme, imidazole buffer rescued activity of a mutant with a cytosine-76 (C76) to uracil substitution. In addition, a C76 to adenine substitution reduced the apparent pKa (where Ka is the acid constant) of the self-cleavage reaction by an amount consistent with differences in the pKa values of these two side chains. These results suggest that, in the wild-type ribozyme, C76 acts as a general base. This finding has implications for potential catalytic functions of conserved cytosines and adenines in other ribozymes and in ribonuclear proteins with enzymatic activity.

Duke Scholars

Published In

Science

DOI

ISSN

0036-8075

Publication Date

October 1, 1999

Volume

286

Issue

5437

Start / End Page

123 / 126

Location

United States

Related Subject Headings

  • Temperature
  • RNA, Catalytic
  • Pyrazoles
  • Protons
  • Point Mutation
  • Mutagenesis
  • Manganese
  • Magnesium Chloride
  • Imidazoles
  • Hydrogen-Ion Concentration
 

Citation

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Perrotta, A. T., Shih, I., & Been, M. D. (1999). Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme. Science, 286(5437), 123–126. https://doi.org/10.1126/science.286.5437.123
Perrotta, A. T., I. Shih, and M. D. Been. “Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme.Science 286, no. 5437 (October 1, 1999): 123–26. https://doi.org/10.1126/science.286.5437.123.
Perrotta AT, Shih I, Been MD. Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme. Science. 1999 Oct 1;286(5437):123–6.
Perrotta, A. T., et al. “Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme.Science, vol. 286, no. 5437, Oct. 1999, pp. 123–26. Pubmed, doi:10.1126/science.286.5437.123.
Perrotta AT, Shih I, Been MD. Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme. Science. 1999 Oct 1;286(5437):123–126.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

October 1, 1999

Volume

286

Issue

5437

Start / End Page

123 / 126

Location

United States

Related Subject Headings

  • Temperature
  • RNA, Catalytic
  • Pyrazoles
  • Protons
  • Point Mutation
  • Mutagenesis
  • Manganese
  • Magnesium Chloride
  • Imidazoles
  • Hydrogen-Ion Concentration