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Tissue-specific alterations in G protein expression in genetic versus diet-induced models of non-insulin-dependent diabetes mellitus in the mouse.

Publication ,  Journal Article
Gettys, TW; Ramkumar, V; Surwit, RS; Taylor, IL
Published in: Metabolism
June 1995

Various tissues were obtained from the well-characterized genetic model (C57BL/6J-ob/ob) of non-insulin-dependent diabetes mellitus (NIDDM) and from a diet-induced model of NIDDM produced in the same genetic background (C57BL/6J). The objectives were to determine whether the previously observed changes in guanine nucleotide-binding regulatory protein (G protein) expression in adipose tissue from ob/ob mice were mirrored by concomitant changes in other tissues, and whether NIDDM of a different etiology would share similar alterations in G protein expression. Plasma membranes from adipocytes, brain, heart, liver, and testes were probed with alpha-subunit-specific antisera, and the level of G protein expression in each model was compared with that in its lean littermate control. Adipose, heart, and liver cell membranes from ob/ob mice contained significantly less alpha-subunit of stimulatory G protein (Gs alpha) than those from their lean littermates. As compared with the lean littermates, heart alpha-subunit-2 of inhibitory G protein (Gi alpha-2), liver Gi alpha-3, and adipocyte G1 alpha-1 and Gi alpha-3 were also reduced in ob/ob mice. In contrast, Gi alpha-2 and Go alpha were increased over lean-control levels in brain tissue from ob/ob mice, whereas Gs alpha was unchanged. G protein expression in the testes did not differ between lean and ob/ob mice. In the diet-induced model of NIDDM, Gs alpha expression in the liver was twofold greater in obese/diabetic mice as compared with lean controls. However, G protein expression in all other tissues examined did not differ between obese/diabetic animals and lean littermates.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Metabolism

DOI

ISSN

0026-0495

Publication Date

June 1995

Volume

44

Issue

6

Start / End Page

771 / 778

Location

United States

Related Subject Headings

  • Reference Values
  • Obesity
  • Mice, Inbred C57BL
  • Mice
  • Male
  • GTP-Binding Proteins
  • Endocrinology & Metabolism
  • Diet
  • Diabetes Mellitus, Type 2
  • Animals
 

Citation

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Gettys, T. W., Ramkumar, V., Surwit, R. S., & Taylor, I. L. (1995). Tissue-specific alterations in G protein expression in genetic versus diet-induced models of non-insulin-dependent diabetes mellitus in the mouse. Metabolism, 44(6), 771–778. https://doi.org/10.1016/0026-0495(95)90191-4
Gettys, T. W., V. Ramkumar, R. S. Surwit, and I. L. Taylor. “Tissue-specific alterations in G protein expression in genetic versus diet-induced models of non-insulin-dependent diabetes mellitus in the mouse.Metabolism 44, no. 6 (June 1995): 771–78. https://doi.org/10.1016/0026-0495(95)90191-4.
Gettys, T. W., et al. “Tissue-specific alterations in G protein expression in genetic versus diet-induced models of non-insulin-dependent diabetes mellitus in the mouse.Metabolism, vol. 44, no. 6, June 1995, pp. 771–78. Pubmed, doi:10.1016/0026-0495(95)90191-4.
Journal cover image

Published In

Metabolism

DOI

ISSN

0026-0495

Publication Date

June 1995

Volume

44

Issue

6

Start / End Page

771 / 778

Location

United States

Related Subject Headings

  • Reference Values
  • Obesity
  • Mice, Inbred C57BL
  • Mice
  • Male
  • GTP-Binding Proteins
  • Endocrinology & Metabolism
  • Diet
  • Diabetes Mellitus, Type 2
  • Animals