Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Developmental regulation of cytochrome oxidase subunit VIa isoforms in cardiac and skeletal muscle.

Publication ,  Journal Article
Parsons, WJ; Williams, RS; Shelton, JM; Luo, Y; Kessler, DJ; Richardson, JA
Published in: Am J Physiol
February 1996

Physiological requirements for mitochondrial respiration change during fetal and postnatal development of cardiac and skeletal muscle, particularly after the abrupt transition from the hypoxic fetal environment to the oxygen-rich milieu of the neonate. This study defines the pattern of expression of nuclear genes encoding the muscle-specific (H) and non-muscle-specific (L) isoforms of cytochrome oxidase (COX) subunit VIa during pre- and postnatal development of striated muscles in the mouse. In the early embryo, COX VIa-L was the predominant isoform expressed in all tissues. COX VIa-H mRNA was detectable as early as day 8 postcoitum (pc) in the heart, but not until gestational day 14 in skeletal myofibers of the tongue, diaphragm, and other skeletal muscles. At late fetal stages up until birth (days 16-18 pc), COX VIa-L and COX VIa-H were both expressed in striated myocytes, although the L form remained the dominant isoform. In postnatal animals, however, expression of COX VIa-H increased whereas COX VIa-L decreased in a reciprocal manner. Activation of the COX VIa-H gene also was observed during differentiation of nurine myogenic cells in culture and was followed by diminished expression of the COX VIa-L isoform in maturing myotubes, as in the intact animal. We conclude that regulation of nuclear genes encoding subunits of COX is a component of the developmental programs that govern cardiac and skeletal muscle differentiation and maturation in the mammalian fetus and neonate. COX VIa-L, the predominant isoform in all fetal tissues, is gradually replaced by the muscle-specific H isoform in both cardiac and skeletal muscles, although this transition is not complete until after birth.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1996

Volume

270

Issue

2 Pt 2

Start / End Page

H567 / H574

Location

United States

Related Subject Headings

  • Rats
  • Myocardium
  • Muscle, Skeletal
  • Molecular Sequence Data
  • Molecular Probes
  • Mice
  • Isoenzymes
  • Heart
  • Embryonic and Fetal Development
  • Electron Transport Complex IV
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Parsons, W. J., Williams, R. S., Shelton, J. M., Luo, Y., Kessler, D. J., & Richardson, J. A. (1996). Developmental regulation of cytochrome oxidase subunit VIa isoforms in cardiac and skeletal muscle. Am J Physiol, 270(2 Pt 2), H567–H574. https://doi.org/10.1152/ajpheart.1996.270.2.H567
Parsons, W. J., R. S. Williams, J. M. Shelton, Y. Luo, D. J. Kessler, and J. A. Richardson. “Developmental regulation of cytochrome oxidase subunit VIa isoforms in cardiac and skeletal muscle.Am J Physiol 270, no. 2 Pt 2 (February 1996): H567–74. https://doi.org/10.1152/ajpheart.1996.270.2.H567.
Parsons WJ, Williams RS, Shelton JM, Luo Y, Kessler DJ, Richardson JA. Developmental regulation of cytochrome oxidase subunit VIa isoforms in cardiac and skeletal muscle. Am J Physiol. 1996 Feb;270(2 Pt 2):H567–74.
Parsons, W. J., et al. “Developmental regulation of cytochrome oxidase subunit VIa isoforms in cardiac and skeletal muscle.Am J Physiol, vol. 270, no. 2 Pt 2, Feb. 1996, pp. H567–74. Pubmed, doi:10.1152/ajpheart.1996.270.2.H567.
Parsons WJ, Williams RS, Shelton JM, Luo Y, Kessler DJ, Richardson JA. Developmental regulation of cytochrome oxidase subunit VIa isoforms in cardiac and skeletal muscle. Am J Physiol. 1996 Feb;270(2 Pt 2):H567–H574.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1996

Volume

270

Issue

2 Pt 2

Start / End Page

H567 / H574

Location

United States

Related Subject Headings

  • Rats
  • Myocardium
  • Muscle, Skeletal
  • Molecular Sequence Data
  • Molecular Probes
  • Mice
  • Isoenzymes
  • Heart
  • Embryonic and Fetal Development
  • Electron Transport Complex IV