Scholars@Duke
Journal cover image

A human protein related to yeast Cdc6p.

Publication ,  Journal Article
Williams, RS; Shohet, RV; Stillman, B
Published in: Proc Natl Acad Sci U S A
January 7, 1997
Published version (DOI) Link to item

The unstable proteins Cdc6p and cdc18+ are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively, and also participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. We have identified Xenopus and human proteins closely related to Cdc6p/cdc18. The human protein, p62(cdc6), is encoded on chromosome 17q21.3 and includes putative cyclin-dependent kinase phosphorylation sites, destruction boxes, a nucleotide binding/ATPase domain, and a potential leucine zipper. Expression of p62(cdc6) mRNA and protein is suppressed in human diploid fibroblasts made quiescent by serum starvation, and peaks as cells reenter the cell cycle and replicate DNA following serum stimulation. Conservation of structure among proteins involved in initiation suggests that fundamental features of replication complexes are maintained in all eukaryotes.

Duke Scholars

Robert Sanders Williams
Author Robert Sanders Williams Medicine, Cardiology
Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.1.142

ISSN

0027-8424

Publication Date

January 7, 1997

Volume

94

Issue

1

Start / End Page

142 / 147

Location

United States

Related Subject Headings

  • Xenopus
  • Transcription, Genetic
  • Species Specificity
  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • Schizosaccharomyces pombe Proteins
  • RNA, Messenger
  • Nuclear Proteins
  • Molecular Sequence Data
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Williams, R. S., Shohet, R. V., & Stillman, B. (1997). A human protein related to yeast Cdc6p. Proc Natl Acad Sci U S A, 94(1), 142–147. https://doi.org/10.1073/pnas.94.1.142
Williams, R. S., R. V. Shohet, and B. Stillman. “A human protein related to yeast Cdc6p.” Proc Natl Acad Sci U S A 94, no. 1 (January 7, 1997): 142–47. https://doi.org/10.1073/pnas.94.1.142.
Williams RS, Shohet RV, Stillman B. A human protein related to yeast Cdc6p. Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):142–7.
Williams, R. S., et al. “A human protein related to yeast Cdc6p.” Proc Natl Acad Sci U S A, vol. 94, no. 1, Jan. 1997, pp. 142–47. Pubmed, doi:10.1073/pnas.94.1.142.
Williams RS, Shohet RV, Stillman B. A human protein related to yeast Cdc6p. Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):142–147.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.1.142

ISSN

0027-8424

Publication Date

January 7, 1997

Volume

94

Issue

1

Start / End Page

142 / 147

Location

United States

Related Subject Headings

  • Xenopus
  • Transcription, Genetic
  • Species Specificity
  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • Schizosaccharomyces pombe Proteins
  • RNA, Messenger
  • Nuclear Proteins
  • Molecular Sequence Data
  • Humans