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Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes.

Publication ,  Journal Article
Neyses, L; Nouskas, J; Luyken, J; Fronhoffs, S; Oberdorf, S; Pfeifer, U; Williams, RS; Sukhatme, VP; Vetter, H
Published in: J Hypertens
September 1993

OBJECTIVE: Few molecular signals for induction of myocardial hypertrophy have been identified. This study was carried out to investigate the action of angiotensin II and endothelin on the growth- and differentiation-related genes Egr-1 (early growth response gene 1) and c-fos in isolated adult rat cardiomyocytes. METHODS: Cardiac myocytes from male Wistar-Kyoto rats were isolated and incubated with angiotensin II and endothelin-1 in Dulbecco's modified Eagle's medium. RNA was isolated and blotted, and densitometric analysis was performed. All experiments were repeated at least three times. RESULTS: Endothelin-1 (10(-7) mmol/l) induced a 20-25-fold rise in Egr-1 messenger RNA within 15 min. This effect was dose-dependent. c-fos was induced 10-20-fold within 15 min with similar dose-response characteristics. Angiotensin II also induced Egr-1 and c-fos with kinetics similar to endothelin but a cofactor from fetal calf serum was needed for full c-fos expression. The protein kinase C activator phorbol 12-myristate 13-acetate also induced Egr-1. CONCLUSIONS: The results identify Egr-1 and c-fos as target genes for the action of endothelin and angiotensin II in the adult myocardium suggesting that induction of the genes may be part of the signal transduction pathway for angiotensin II and endothelin in the myocardium.

Duke Scholars

Published In

J Hypertens

DOI

ISSN

0263-6352

Publication Date

September 1993

Volume

11

Issue

9

Start / End Page

927 / 934

Location

Netherlands

Related Subject Headings

  • Transcription Factors
  • Rats, Inbred WKY
  • Rats
  • RNA
  • Myocardium
  • Male
  • Immediate-Early Proteins
  • Genes, fos
  • Genes, Immediate-Early
  • Gene Expression Regulation
 

Citation

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MLA
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Neyses, L., Nouskas, J., Luyken, J., Fronhoffs, S., Oberdorf, S., Pfeifer, U., … Vetter, H. (1993). Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes. J Hypertens, 11(9), 927–934. https://doi.org/10.1097/00004872-199309000-00006
Neyses, L., J. Nouskas, J. Luyken, S. Fronhoffs, S. Oberdorf, U. Pfeifer, R. S. Williams, V. P. Sukhatme, and H. Vetter. “Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes.J Hypertens 11, no. 9 (September 1993): 927–34. https://doi.org/10.1097/00004872-199309000-00006.
Neyses L, Nouskas J, Luyken J, Fronhoffs S, Oberdorf S, Pfeifer U, et al. Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes. J Hypertens. 1993 Sep;11(9):927–34.
Neyses, L., et al. “Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes.J Hypertens, vol. 11, no. 9, Sept. 1993, pp. 927–34. Pubmed, doi:10.1097/00004872-199309000-00006.
Neyses L, Nouskas J, Luyken J, Fronhoffs S, Oberdorf S, Pfeifer U, Williams RS, Sukhatme VP, Vetter H. Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes. J Hypertens. 1993 Sep;11(9):927–934.

Published In

J Hypertens

DOI

ISSN

0263-6352

Publication Date

September 1993

Volume

11

Issue

9

Start / End Page

927 / 934

Location

Netherlands

Related Subject Headings

  • Transcription Factors
  • Rats, Inbred WKY
  • Rats
  • RNA
  • Myocardium
  • Male
  • Immediate-Early Proteins
  • Genes, fos
  • Genes, Immediate-Early
  • Gene Expression Regulation