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Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins.

Publication ,  Journal Article
Benson, DE; Conrad, DW; de Lorimier, RM; Trammell, SA; Hellinga, HW
Published in: Science
August 31, 2001

We report a flexible strategy for transducing ligand-binding events into electrochemical responses for a wide variety of proteins. The method exploits ligand-mediated hinge-bending motions, intrinsic to the bacterial periplasmic binding protein superfamily, to establish allosterically controlled interactions between electrode surfaces and redox-active, Ru(II)-labeled proteins. This approach allows the development of protein-based bioelectronic interfaces that respond to a diverse set of analytes. Families of these interfaces can be generated either by exploiting natural binding diversity within the superfamily or by reengineering the specificity of individual proteins. These proteins may have numerous medical, environmental, and defense applications.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

August 31, 2001

Volume

293

Issue

5535

Start / End Page

1641 / 1644

Location

United States

Related Subject Headings

  • Zinc
  • Thermodynamics
  • Signal Transduction
  • Ruthenium
  • Rats
  • Protein Engineering
  • Protein Conformation
  • Oxidation-Reduction
  • Mutation
  • Monosaccharide Transport Proteins
 

Citation

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Benson, D. E., Conrad, D. W., de Lorimier, R. M., Trammell, S. A., & Hellinga, H. W. (2001). Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins. Science, 293(5535), 1641–1644. https://doi.org/10.1126/science.1062461
Benson, D. E., D. W. Conrad, R. M. de Lorimier, S. A. Trammell, and H. W. Hellinga. “Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins.Science 293, no. 5535 (August 31, 2001): 1641–44. https://doi.org/10.1126/science.1062461.
Benson DE, Conrad DW, de Lorimier RM, Trammell SA, Hellinga HW. Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins. Science. 2001 Aug 31;293(5535):1641–4.
Benson, D. E., et al. “Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins.Science, vol. 293, no. 5535, Aug. 2001, pp. 1641–44. Pubmed, doi:10.1126/science.1062461.
Benson DE, Conrad DW, de Lorimier RM, Trammell SA, Hellinga HW. Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins. Science. 2001 Aug 31;293(5535):1641–1644.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

August 31, 2001

Volume

293

Issue

5535

Start / End Page

1641 / 1644

Location

United States

Related Subject Headings

  • Zinc
  • Thermodynamics
  • Signal Transduction
  • Ruthenium
  • Rats
  • Protein Engineering
  • Protein Conformation
  • Oxidation-Reduction
  • Mutation
  • Monosaccharide Transport Proteins