Skip to main content
release_alert
Welcome to the new Scholars 3.0! Read about new features and let us know what you think.
cancel
Journal cover image

Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini.

Publication ,  Journal Article
Liddle, RA; Elashoff, J; Reeve, JR
Published in: Peptides
1986

The relative potencies of cholecystokinin (CCK-33) and its carboxyl terminal octapeptide (CCK-8) for stimulation of amylase release from rat pancreatic acini was measured. Porcine CCK-33 and synthetic CCK-8 were initially subjected to high pressure liquid chromatography to assess purity. Concentrations of each peptide were determined by amino acid analysis. The relative immunoreactivities of CCK-33 and CCK-8 were compared using an antibody that recognizes the common carboxyl terminus of these forms. This antibody bound CCK-8 and CCK-33 with nearly equal affinity. The relative potencies of CCK-33 and CCK-8 were then measured by comparing their abilities to stimulate amylase release from isolated rat pancreatic acini. Statistical analysis of the relative potencies of the two hormones indicated that CCK-8 was 36% more potent than CCK-33 in this assay system. These data suggest that differences in biological activities between large and small forms of CCK are not as great as previously reported.

Duke Scholars

Published In

Peptides

DOI

ISSN

0196-9781

Publication Date

1986

Volume

7

Issue

5

Start / End Page

723 / 727

Location

United States

Related Subject Headings

  • Sincalide
  • Rats
  • Pancreatic Juice
  • Pancreas
  • Cholecystokinin
  • Animals
  • Amylases
  • Amino Acids
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liddle, R. A., Elashoff, J., & Reeve, J. R. (1986). Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini. Peptides, 7(5), 723–727. https://doi.org/10.1016/0196-9781(86)90085-9
Liddle, R. A., J. Elashoff, and J. R. Reeve. “Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini.Peptides 7, no. 5 (1986): 723–27. https://doi.org/10.1016/0196-9781(86)90085-9.
Liddle RA, Elashoff J, Reeve JR. Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini. Peptides. 1986;7(5):723–7.
Liddle, R. A., et al. “Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini.Peptides, vol. 7, no. 5, 1986, pp. 723–27. Pubmed, doi:10.1016/0196-9781(86)90085-9.
Liddle RA, Elashoff J, Reeve JR. Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini. Peptides. 1986;7(5):723–727.
Journal cover image

Published In

Peptides

DOI

ISSN

0196-9781

Publication Date

1986

Volume

7

Issue

5

Start / End Page

723 / 727

Location

United States

Related Subject Headings

  • Sincalide
  • Rats
  • Pancreatic Juice
  • Pancreas
  • Cholecystokinin
  • Animals
  • Amylases
  • Amino Acids