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The 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain.

Publication ,  Journal Article
Rudolph, MJ; Johnson, JL; Rajagopalan, KV; Kisker, C
Published in: Acta Crystallogr D Biol Crystallogr
July 2003

The molybdenum- and iron-containing enzyme sulfite oxidase catalyzes the physiologically vital oxidation of sulfite to sulfate. Sulfite oxidase contains three domains: an N-terminal cytochrome b(5) domain, a central domain harboring the molybdenum cofactor (Moco) and a C-terminal dimerization domain. Oxidation of the substrate sulfite is coupled to the transfer of two electrons to the molybdenum cofactor. Subsequently, these electrons are passed on, one at a time, to the b(5) heme of sulfite oxidase and from there to the soluble electron carrier cytochrome c. The crystal structure of the oxidized human sulfite oxidase cytochrome b(5) domain has been determined at 1.2 A resolution and has been refined to a crystallographic R factor of 0.107 (R(free) = 0.137). A comparison of this structure with other b(5)-type cytochromes reveals distinct structural features present in the sulfite oxidase b(5) domain which promote optimal electron transport between the Moco of sulfite oxidase and the heme of cytochrome c.

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Published In

Acta Crystallogr D Biol Crystallogr

DOI

ISSN

0907-4449

Publication Date

July 2003

Volume

59

Issue

Pt 7

Start / End Page

1183 / 1191

Location

United States

Related Subject Headings

  • Thermodynamics
  • Static Electricity
  • Sequence Alignment
  • Protein Structure, Tertiary
  • Protein Conformation
  • Oxidoreductases Acting on Sulfur Group Donors
  • Humans
  • Electron Transport
  • Cytochromes b5
  • Crystallography, X-Ray
 

Citation

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Rudolph, M. J., Johnson, J. L., Rajagopalan, K. V., & Kisker, C. (2003). The 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain. Acta Crystallogr D Biol Crystallogr, 59(Pt 7), 1183–1191. https://doi.org/10.1107/s0907444903009934
Rudolph, Michael J., Jean L. Johnson, K. V. Rajagopalan, and Caroline Kisker. “The 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain.Acta Crystallogr D Biol Crystallogr 59, no. Pt 7 (July 2003): 1183–91. https://doi.org/10.1107/s0907444903009934.
Rudolph MJ, Johnson JL, Rajagopalan KV, Kisker C. The 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain. Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1183–91.
Rudolph, Michael J., et al. “The 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain.Acta Crystallogr D Biol Crystallogr, vol. 59, no. Pt 7, July 2003, pp. 1183–91. Pubmed, doi:10.1107/s0907444903009934.
Rudolph MJ, Johnson JL, Rajagopalan KV, Kisker C. The 1.2 A structure of the human sulfite oxidase cytochrome b(5) domain. Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1183–1191.
Journal cover image

Published In

Acta Crystallogr D Biol Crystallogr

DOI

ISSN

0907-4449

Publication Date

July 2003

Volume

59

Issue

Pt 7

Start / End Page

1183 / 1191

Location

United States

Related Subject Headings

  • Thermodynamics
  • Static Electricity
  • Sequence Alignment
  • Protein Structure, Tertiary
  • Protein Conformation
  • Oxidoreductases Acting on Sulfur Group Donors
  • Humans
  • Electron Transport
  • Cytochromes b5
  • Crystallography, X-Ray